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dc.contributor.author
Delmotte, Nathanaël
dc.contributor.author
Mondy, Samuel
dc.contributor.author
Alunni, Benoit
dc.contributor.author
Fardoux, Joel
dc.contributor.author
Chaintreuil, Clémence
dc.contributor.author
Vorholt, Julia A.
dc.contributor.author
Giraud, Eric
dc.contributor.author
Gourion, Benjamin
dc.date.accessioned
2018-11-02T12:38:25Z
dc.date.available
2017-06-11T14:59:11Z
dc.date.available
2018-11-02T12:38:25Z
dc.date.issued
2014-02-28
dc.identifier.issn
1422-0067
dc.identifier.other
10.3390/ijms15033660
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/95139
dc.identifier.doi
10.3929/ethz-b-000095139
dc.description.abstract
Rhizobia are soil bacteria that are able to form symbiosis with plant hosts of the legume family. These associations result in the formation of organs, called nodules in which bacteria fix atmospheric nitrogen to the benefit of the plant. Most of our knowledge on the metabolism and the physiology of the bacteria during symbiosis derives from studying roots nodules of terrestrial plants. Here we used a proteomics approach to investigate the bacterial physiology of photosynthetic Bradyrhizobium sp. ORS278 during the symbiotic process with the semi aquatical plant Aeschynomene indica that forms root and stem nodules. We analyzed the proteomes of bacteria extracted from each type of nodule. First, we analyzed the bacteroid proteome at two different time points and found only minor variation between the bacterial proteomes of 2-week- and 3-week-old nodules. High conservation of the bacteroid proteome was also found when comparing stem nodules and root nodules. Among the stem nodule specific proteins were those related to the phototrophic ability of Bradyrhizobium sp. ORS278. Furthermore, we compared our data with those obtained during an extensive genetic screen previously published. The symbiotic role of four candidate genes which corresponding proteins were found massively produced in the nodules but not identified during this screening was examined. Mutant analysis suggested that in addition to the EtfAB system, the fixA locus is required for symbiotic efficiency.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
MDPI
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/3.0/
dc.subject
Proteome
en_US
dc.subject
Photosynthetic Bradyrhizobium
en_US
dc.subject
Symbiotic nitrogen fixation
en_US
dc.subject
Stem nodulation
en_US
dc.subject
fixA
en_US
dc.title
A Proteomic Approach of Bradyrhizobium/Aeschynomene Root and Stem Symbioses Reveals the Importance of the fixA Locus for Symbiosis
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported
ethz.journal.title
International Journal of Molecular Sciences
ethz.journal.volume
15
en_US
ethz.journal.issue
3
en_US
ethz.journal.abbreviated
Int. j. mol. sci.
ethz.pages.start
3660
en_US
ethz.pages.end
3670
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
004068146
ethz.publication.place
S.l.
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03740 - Vorholt, Julia / Vorholt, Julia
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02520 - Institut für Mikrobiologie / Institute of Microbiology::03740 - Vorholt, Julia / Vorholt, Julia
ethz.date.deposited
2017-06-11T15:00:00Z
ethz.source
ECIT
ethz.identifier.importid
imp593652b92e32758988
ethz.ecitpid
pub:149314
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-20T17:21:40Z
ethz.rosetta.lastUpdated
2018-11-02T12:38:38Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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