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dc.contributor.author
Arranz, Alicia
dc.contributor.author
Dong, Di
dc.contributor.author
Zhu, Shouping Zhu
dc.contributor.author
Savakis, Charalambos
dc.contributor.author
Tian, Jie
dc.contributor.author
Ripoll, Jorge
dc.date.accessioned
2018-10-12T15:56:20Z
dc.date.available
2017-06-11T15:20:27Z
dc.date.available
2018-10-12T15:56:20Z
dc.date.issued
2014-12-04
dc.identifier.other
10.1038/srep07325
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/96063
dc.identifier.doi
10.3929/ethz-b-000096063
dc.description.abstract
Even though in vivo imaging approaches have witnessed several new and important developments, specimens that exhibit high light scattering properties such as Drosophila melanogaster pupae are still not easily accessible with current optical imaging techniques, obtaining images only from subsurface features. This means that in order to obtain 3D volumetric information these specimens need to be studied either after fixation and a chemical clearing process, through an imaging window - thus perturbing physiological development -, or during early stages of development when the scattering contribution is negligible. In this paper we showcase how Optical Projection Tomography may be used to obtain volumetric images of the head eversion process in vivo in Drosophila melanogaster pupae, both in control and headless mutant specimens. Additionally, we demonstrate the use of Helical Optical Projection Tomography (hOPT) as a tool for high throughput 4D-imaging of several specimens simultaneously.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Nature Publishing Group
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc-sa/4.0/
dc.subject
Time-lapse imaging
en_US
dc.subject
Biological metamorphosis
en_US
dc.title
In-vivo Optical Tomography of Small Scattering Specimens
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International
ethz.title.subtitle
time-lapse 3D imaging of the head eversion process in Drosophila melanogaster
en_US
ethz.journal.title
Scientific Reports
ethz.journal.volume
4
en_US
ethz.pages.start
7325
en_US
ethz.size
5p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
006751867
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-11T15:20:45Z
ethz.source
ECIT
ethz.identifier.importid
imp593652ca22ac281865
ethz.ecitpid
pub:150634
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-31T16:31:36Z
ethz.rosetta.lastUpdated
2019-01-02T14:31:55Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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