Measuring membrane channel dynamics in microfluidics: A future alternative to patch clamping?
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We present a novel analytical strategy for the measurement of membrane channel dynamics involved in cellular mechanotransduction signaling pathways. It consists of a conventional PDMS microfluidic device for the culture and mechanical actuation of C2C12 murine myoblasts. Herein, fluid-flow induced shear forces stimulate stretch-activated channels (SAC) of the transient receptor potential (TRP) class that mediate Ca2+ entry. Compared to conventional electrophysiological technology for SAC characterization, our strategy is capable of non-invasively monitoring SACs at high throughput and facilitates the study of the effect of extraneous factors on channel activity in complex media. Show more
Book title18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)
Pages / Article No.
PublisherChemical and Biological Microsystems Society
SubjectMechanobiology; Shear Stress; Calcium; Transient Receptor Potential Channels; C2C12; Myogenesis
Organisational unit09597 - Würtz, Karin (SNF-Professur) (ehemalig) / Würtz, Karin (SNF-Professur) (former)
03915 - Ferguson, Stephen / Ferguson, Stephen
03807 - Dittrich, Petra / Dittrich, Petra
NotesPoster abstract. Poster presentation on October 28, 2014.
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