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dc.contributor.author
Roos, Martina
dc.contributor.author
Rebhan, Mario A.E.
dc.contributor.author
Lucic, Matije
dc.contributor.author
Pavlicek, David
dc.contributor.author
Pradère, Ugo
dc.contributor.author
Towbin, Harry
dc.contributor.author
Civenni, Gianluca
dc.contributor.author
Catapano, Carlo V.
dc.contributor.author
Hall, Jonathan
dc.date.accessioned
2019-04-04T12:08:10Z
dc.date.available
2017-06-11T15:25:18Z
dc.date.available
2019-04-04T12:08:10Z
dc.date.issued
2015-01-30
dc.identifier.issn
1362-4962
dc.identifier.issn
0305-1048
dc.identifier.issn
1362-4954
dc.identifier.other
10.1093/nar/gku1090
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/96211
dc.identifier.doi
10.3929/ethz-b-000096211
dc.description.abstract
MicroRNAs (miRNAs) originate from stem-loop-containing precursors (pre-miRNAs, pri-miRNAs) and mature by means of the Drosha and Dicer endonucleases and their associated factors. The let-7 miRNAs have prominent roles in developmental differentiation and in regulating cell proliferation. In cancer, the tumor suppressor function of let-7 is abrogated by overexpression of Lin28, one of several RNA-binding proteins that regulate let-7 biogenesis by interacting with conserved motifs in let-7 precursors close to the Dicer cleavage site. Using in vitro assays, we have identified a binding site for short modified oligoribonucleotides (‘looptomirs’) overlapping that of Lin28 in pre-let-7a-2. These looptomirs selectively antagonize the docking of Lin28, but still permit processing of pre-let-7a-2 by Dicer. Looptomirs restored synthesis of mature let-7 and inhibited growth and clonogenic potential in Lin28 overexpressing hepatocarcinoma cells, thereby demonstrating a promising new means to rescue defective miRNA biogenesis in Lin28-dependent cancers.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Oxford University Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by-nc/4.0/
dc.title
Short loop-targeting oligoribonucleotides antagonize Lin28 and enable pre-let-7 processing and suppression of cell growth in let-7-deficient cancer cells
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution-NonCommercial 4.0 International
dc.date.published
2014-11-06
ethz.journal.title
Nucleic Acids Research
ethz.journal.volume
43
en_US
ethz.journal.issue
2
en_US
ethz.journal.abbreviated
Nucleic Acids Res.
ethz.pages.start
e9
en_US
ethz.size
11 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
000038633
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03760 - Hall, Jonathan / Hall, Jonathan
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02020 - Dep. Chemie und Angewandte Biowiss. / Dep. of Chemistry and Applied Biosc.::02534 - Institut für Pharmazeutische Wiss. / Institute of Pharmaceutical Sciences::03760 - Hall, Jonathan / Hall, Jonathan
ethz.date.deposited
2017-06-11T15:26:05Z
ethz.source
ECIT
ethz.identifier.importid
imp593652cce754f61277
ethz.ecitpid
pub:150804
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-20T15:16:39Z
ethz.rosetta.lastUpdated
2019-04-04T12:08:15Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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