Show simple item record

dc.contributor.author
Dempsey, William P.
dc.contributor.author
Qin, Hanyu
dc.contributor.author
Pantazis, Periklis
dc.contributor.editor
Cambridge, Sidney
dc.date.accessioned
2021-07-13T14:16:16Z
dc.date.available
2017-06-11T15:35:31Z
dc.date.available
2021-07-13T14:16:16Z
dc.date.issued
2014
dc.identifier.isbn
978-1-4939-0469-3
en_US
dc.identifier.isbn
978-1-4939-0470-9
en_US
dc.identifier.issn
1064-3745
dc.identifier.issn
1940-6029
dc.identifier.other
10.1007/978-1-4939-0470-9_14
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/96645
dc.description.abstract
By combining the strength of previously described in vivo cell tracking methodologies, we have recently generated a set of transgenic zebrafish lines, called “PhOTO (photoconvertible optical tracking of…)” zebrafish. PhOTO zebrafish lines are suitable for cell tracking during highly dynamic events, including gastrulation, tissue regeneration, tumorigenesis, and cancer/disease progression. Global monitoring of cell shape, cell interactions, e.g., cell intercalations, coordinated division, and cell dynamics are accomplished by using fluorescence imaging of nuclear and plasma membrane fluorescent protein labeling. The irreversible green-to-red photoconversion property of Dendra2 fusions enables noninvasive, specific and high-contrast selection of targeted cells of interest, which greatly simplifies cell tracking and segmentation in time and space. Here we demonstrate photoconversion and in vivo cell tracking using PhOTO zebrafish.
en_US
dc.language.iso
en
en_US
dc.publisher
Humana Press
en_US
dc.subject
Photoconversion
en_US
dc.subject
Dendra2
en_US
dc.subject
Cerulean
en_US
dc.subject
Lineage tracing
en_US
dc.subject
Segmentation
en_US
dc.title
In Vivo Cell Tracking Using PhOTO Zebrafish
en_US
dc.type
Book Chapter
dc.date.published
2014-03-21
ethz.book.title
Photoswitching Proteins
en_US
ethz.journal.title
Methods in Molecular Biology
ethz.journal.volume
1148
en_US
ethz.journal.abbreviated
Methods Mol Biol
ethz.pages.start
217
en_US
ethz.pages.end
228
en_US
ethz.identifier.nebis
000916990
ethz.publication.place
New York, NY
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03945 - Pantazis, Periklis (ehemalig)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03945 - Pantazis, Periklis (ehemalig)
ethz.date.deposited
2017-06-11T15:35:51Z
ethz.source
ECIT
ethz.identifier.importid
imp593652d45eb5230328
ethz.ecitpid
pub:151327
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2017-07-13T17:24:51Z
ethz.rosetta.lastUpdated
2022-03-29T10:23:38Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=In%20Vivo%20Cell%20Tracking%20Using%20PhOTO%20Zebrafish&rft.jtitle=Methods%20in%20Molecular%20Biology&rft.date=2014&rft.volume=1148&rft.spage=217&rft.epage=228&rft.issn=1064-3745&1940-6029&rft.au=Dempsey,%20William%20P.&Qin,%20Hanyu&Pantazis,%20Periklis&rft.isbn=978-1-4939-0469-3&978-1-4939-0470-9&rft.genre=bookitem&rft_id=info:doi/10.1007/978-1-4939-0470-9_14&rft.btitle=Photoswitching%20Proteins
 Search print copy at ETH Library

Files in this item

FilesSizeFormatOpen in viewer

There are no files associated with this item.

Publication type

Show simple item record