Monitoring of 3D multi-cellular spheroids in hanging drop networks through in-situ impedance spectroscopy
- Other Conference Item
We present the integration of electrical impedance spectroscopy (EIS) into a microfluidic network of hanging drops, in which spherical microtissues can be formed and cultured over time. EIS offers continuous monitoring of the drop volume and media conductivity and in-situ measurements of spheroid growth for different culturing conditions. The integration has been achieved by using a hybrid fabrication method, in which electrode modules on glass substrates were embedded into a microfluidic PDMS structure. The measurement results show robust drop volume control and, for the first time, size determination of human colon carcinoma spheroids directly inside a hanging drop. Show more
Book title18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)
Pages / Article No.
PublisherChemical and Biological Microsystems Society
SubjectHanging drop; Spheroid; Impedance; 3D tissue
Organisational unit03684 - Hierlemann, Andreas / Hierlemann, Andreas
296257 - The Body-on-a-Chip (BoC) (EC)
142440 - The Body on a Chip - Human 3-D tissue analogues in microfluidic systems (SNF)
NotesExtended abstract. Conference lecture on October, 28, 2014.
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