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dc.contributor.author
Palma-Guerrero, Javier
dc.contributor.author
Hall, Charles R.
dc.contributor.author
Kowbel, David
dc.contributor.author
Welch, Juliet
dc.contributor.author
Taylor, John W.
dc.contributor.author
Brem, Rachel B.
dc.contributor.author
Glass, N. Louise
dc.date.accessioned
2018-10-31T16:05:35Z
dc.date.available
2017-06-11T15:48:55Z
dc.date.available
2018-10-31T16:05:35Z
dc.date.issued
2013-08-01
dc.identifier.issn
1553-7390
dc.identifier.issn
1553-7404
dc.identifier.other
10.1371/journal.pgen.1003669
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/97261
dc.identifier.doi
10.3929/ethz-b-000097261
dc.description.abstract
Understanding how genomes encode complex cellular and organismal behaviors has become the outstanding challenge of modern genetics. Unlike classical screening methods, analysis of genetic variation that occurs naturally in wild populations can enable rapid, genome-scale mapping of genotype to phenotype with a medium-throughput experimental design. Here we describe the results of the first genome-wide association study (GWAS) used to identify novel loci underlying trait variation in a microbial eukaryote, harnessing wild isolates of the filamentous fungus Neurospora crassa. We genotyped each of a population of wild Louisiana strains at 1 million genetic loci genome-wide, and we used these genotypes to map genetic determinants of microbial communication. In N. crassa, germinated asexual spores (germlings) sense the presence of other germlings, grow toward them in a coordinated fashion, and fuse. We evaluated germlings of each strain for their ability to chemically sense, chemotropically seek, and undergo cell fusion, and we subjected these trait measurements to GWAS. This analysis identified one gene, NCU04379 (cse-1, encoding a homolog of a neuronal calcium sensor), at which inheritance was strongly associated with the efficiency of germling communication. Deletion of cse-1 significantly impaired germling communication and fusion, and two genes encoding predicted interaction partners of CSE1 were also required for the communication trait. Additionally, mining our association results for signaling and secretion genes with a potential role in germling communication, we validated six more previously unknown molecular players, including a secreted protease and two other genes whose deletion conferred a novel phenotype of increased communication and multi-germling fusion. Our results establish protein secretion as a linchpin of germling communication in N. crassa and shed light on the regulation of communication molecules in this fungus. Our study demonstrates the power of population-genetic analyses for the rapid identification of genes contributing to complex traits in microbial species.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science (PLoS)
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Genome Wide Association identifies novel loci involved in fungal communication
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
PLoS Genetics
ethz.journal.volume
9
en_US
ethz.journal.issue
8
en_US
ethz.journal.abbreviated
PLoS Genet
ethz.pages.start
e1003669
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
005410305
ethz.publication.place
San Francisco, CA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03516 - McDonald, Bruce / McDonald, Bruce
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02350 - Dep. Umweltsystemwissenschaften / Dep. of Environmental Systems Science::02720 - Institut für Integrative Biologie / Institute of Integrative Biology::03516 - McDonald, Bruce / McDonald, Bruce
ethz.date.deposited
2017-06-11T15:49:01Z
ethz.source
ECIT
ethz.identifier.importid
imp593652de8b60746190
ethz.ecitpid
pub:152189
ethz.eth
no
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-14T20:29:51Z
ethz.rosetta.lastUpdated
2018-10-31T16:05:49Z
ethz.rosetta.versionExported
true
ethz.COinS
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