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dc.contributor.author
El-Warrak, Alexander O.
dc.contributor.author
Olmstead, Marvin
dc.contributor.author
Schneider, Rebecca
dc.contributor.author
Meinel, Lorenz
dc.contributor.author
Bettschart-Wolfisberger, Regula
dc.contributor.author
Akens, Margarete K.
dc.contributor.author
Auer, Jörg
dc.contributor.author
von Rechenberg, Brigitte
dc.date.accessioned
2019-05-13T11:34:26Z
dc.date.available
2017-06-11T16:11:56Z
dc.date.available
2019-05-13T10:11:04Z
dc.date.available
2019-05-13T10:11:50Z
dc.date.available
2019-05-13T11:34:26Z
dc.date.issued
2004
dc.identifier.issn
1471-2474
dc.identifier.other
10.1186/1471-2474-5-7
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/98205
dc.identifier.doi
10.3929/ethz-b-000098205
dc.description.abstract
Background Aseptic loosening of hip prosthesis as it occurs in clinical cases in human patients was attributed to wear particles of the implants, the response of the tissue dominated by macrophages and the production of inflammatory mediators and matrix degrading enzymes; however, the cascade of events initiating the process and their interaction regarding the time course is still open and discussed controversially. Therefore, the goal of this study was to establish an experimental animal model in sheep allowing to follow the cascade of early mechanical and biochemical events within the interface membrane and study the sequence of how they contribute to the pathological bone resorption necessary for aseptic loosening of the implant. Methods A cemented modular system (Biomedtrix) was used as a hip replacement in 24 adult Swiss Alpine sheep, with one group receiving a complete cement mantle as controls (n = 12), and the other group a cement mantle with a standardized, lateral, primary defect in the cement mantle (n = 12). Animals were followed over time for 2 and 8.5 months (n = 6 each). After sacrifice, samples from the interface membranes were harvested from five different regions of the femur and joint capsule. Explant cell cultures were performed and supernatant of cultures were tested and assayed for nitric oxide, prostaglandin E2, caseinolytic and collagenolytic activity. RNA extraction and quantification were performed for inducible nitric oxide synthase, cyclooxygenase-2, interleukin 1, and interleukin 6. Overall differences between groups and time periods and interactions thereof were calculated using a factorial analysis of variance (ANOVA). Results The development of an interface membrane was noticed in both groups at both time points. However, in the controls the interface membrane regressed in thickness and biological activity, while both variables increased in the experimental group with the primary cement mantle defect over time. Nitric oxide (NO) and PGE2 concentrations were higher in the 8.5 months group (P < 0.0001) compared to the 2 months group with a tendency for the unstable group to have higher concentrations. The same was true for collagenolytic activity (P = 0.05), but not for caseinolytic activity that decreased over time (P < 0.0001). Conclusion In this study, a primary cement mantle defect of the femoral shaft elicited biomechanical instability and biochemical changes over time in an experimental animal study in sheep, that resembled the changes described at the bone cement-interface in aseptic loosening of total hip prosthesis in humans. The early biochemical changes may well explain the pathologic bone resorption and formation of an interface membrane as is observed in clinical cases. This animal model may aid in future studies aiming at prevention of aseptic loosening of hip prosthesis and reflect some aspects of the pathogenesis involved.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
BioMed Central
en_US
dc.rights.uri
http://rightsstatements.org/page/InC-NC/1.0/
dc.subject
Nitric Oxide
en_US
dc.subject
PGE2
en_US
dc.subject
Femoral Shaft
en_US
dc.subject
Aseptic Loosening
en_US
dc.subject
Cement Mantle
en_US
dc.title
An experimental animal model of aseptic loosening of hip prostheses in sheep to study early biochemical changes at the interface membrane
en_US
dc.type
Journal Article
dc.rights.license
In Copyright - Non-Commercial Use Permitted
dc.date.published
2004-03-03
ethz.journal.title
BMC Musculoskeletal Disorders
ethz.journal.volume
5
en_US
ethz.journal.abbreviated
BMC musculoskelet. disord. (Online)
ethz.pages.start
7
en_US
ethz.size
13 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.nebis
008594042
ethz.publication.place
London
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02130 - Dep. Maschinenbau und Verfahrenstechnik / Dep. of Mechanical and Process Eng.::02629 - Institut für Verfahrenstechnik / Institute of Process Engineering::03484 - Mazzotti, Marco / Mazzotti, Marco
en_US
ethz.leitzahl
03282 - Merkle, Hans Peter
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02130 - Dep. Maschinenbau und Verfahrenstechnik / Dep. of Mechanical and Process Eng.::02629 - Institut für Verfahrenstechnik / Institute of Process Engineering::03484 - Mazzotti, Marco / Mazzotti, Marco
ethz.leitzahl.certified
03282 - Merkle, Hans Peter
ethz.date.deposited
2017-06-11T16:12:20Z
ethz.source
ECIT
ethz.identifier.importid
imp593652efa6a9858601
ethz.ecitpid
pub:153521
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-31T15:20:18Z
ethz.rosetta.lastUpdated
2019-05-13T11:34:36Z
ethz.rosetta.exportRequired
true
ethz.rosetta.versionExported
true
ethz.COinS
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