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dc.contributor.author
Narduzzi-Wicht, Barbara
dc.contributor.author
Jermini, Mauro
dc.contributor.author
Gessler, Cesare
dc.contributor.author
Broggini, Giovanni A.L.
dc.date.accessioned
2019-10-14T10:06:44Z
dc.date.available
2017-06-11T16:22:02Z
dc.date.available
2019-10-14T10:06:44Z
dc.date.issued
2014
dc.identifier.issn
0031-9465
dc.identifier.issn
1593-2095
dc.identifier.other
10.14601/Phytopathol_Mediterr-14481
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/98607
dc.identifier.doi
10.3929/ethz-b-000098607
dc.description.abstract
Grape black rot, caused by the homothallic ascomycete Phyllosticta ampelicida, is a disease originating from North America and is widespread in Europe. To investigate population structure and epidemics of this pathogen, we developed 11 microsatellite markers. A multiplex PCR assay was used to amplify genomic DNA from environmental samples including mummified berries and foliar lesions, and from fungal cultures. Environmental samples were collected from five countries (Switzerland, France, Germany, Luxembourg and the USA), and consisted of 64 different genotypes. Five additional genotypes were identified from pure cultures isolated in Switzerland and Germany. The allele rarefaction approach indicated that French vineyards in the region of Bordeaux displayed the greatest mean allelic richness, probably related to the fact that France is the country where the disease was first reported outside North America, in 1885. Our results also suggest the absence of links between the species/cultivar of Vitis hosts and the infecting P. ampelicida genotypes. This is the first report of development of microsatellite markers and their deployment for population studies of P. ampelicida.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Mediterranean Phytopathological Union
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.subject
Guignardia bidwellii
en_US
dc.subject
SSR
en_US
dc.subject
population genetics
en_US
dc.subject
population structure
en_US
dc.title
Microsatellite markers for population studies of the ascomycete Phyllosticta ampelicida, the pathogen causing grape black rot
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
dc.date.published
2014-12
ethz.journal.title
Phytopathologia mediterranea
ethz.journal.volume
53
en_US
ethz.journal.issue
3
en_US
ethz.journal.abbreviated
Phytopathol. Mediterr. (Testo stamp.)
ethz.pages.start
470
en_US
ethz.pages.end
479
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.nebis
000008197
ethz.publication.place
Firenze
en_US
ethz.publication.status
published
en_US
ethz.date.deposited
2017-06-11T16:22:20Z
ethz.source
ECIT
ethz.identifier.importid
imp593652f87ddcb75600
ethz.ecitpid
pub:154227
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-18T13:19:25Z
ethz.rosetta.lastUpdated
2019-10-14T10:07:00Z
ethz.rosetta.versionExported
true
ethz.COinS
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