Show simple item record

dc.contributor.author
Scarfone, Ilaria
dc.contributor.author
Venturetti, Marianna
dc.contributor.author
Hotz, Manuel
dc.contributor.author
Lengefeld, Jette
dc.contributor.author
Barral, Yves
dc.contributor.author
Piatti, Simonetta
dc.date.accessioned
2018-11-05T10:38:20Z
dc.date.available
2017-06-11T16:48:23Z
dc.date.available
2018-11-05T10:38:20Z
dc.date.issued
2015-02-06
dc.identifier.issn
1553-7390
dc.identifier.issn
1553-7404
dc.identifier.other
10.1371/journal.pgen.1004938
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/99673
dc.identifier.doi
10.3929/ethz-b-000099673
dc.description.abstract
The asymmetrically dividing yeast S. cerevisiae assembles a bipolar spindle well after establishing the future site of cell division (i.e., the bud neck) and the division axis (i.e., the mother-bud axis). A surveillance mechanism called spindle position checkpoint (SPOC) delays mitotic exit and cytokinesis until the spindle is properly positioned relative to the mother-bud axis, thereby ensuring the correct ploidy of the progeny. SPOC relies on the heterodimeric GTPase-activating protein Bub2/Bfa1 that inhibits the small GTPase Tem1, in turn essential for activating the mitotic exit network (MEN) kinase cascade and cytokinesis. The Bub2/Bfa1 GAP and the Tem1 GTPase form a complex at spindle poles that undergoes a remarkable asymmetry during mitosis when the spindle is properly positioned, with the complex accumulating on the bud-directed old spindle pole. In contrast, the complex remains symmetrically localized on both poles of misaligned spindles. The mechanism driving asymmetry of Bub2/Bfa1/Tem1 in mitosis is unclear. Furthermore, whether asymmetry is involved in timely mitotic exit is controversial. We investigated the mechanism by which the GAP Bub2/Bfa1 controls GTP hydrolysis on Tem1 and generated a series of mutants leading to constitutive Tem1 activation. These mutants are SPOC-defective and invariably lead to symmetrical localization of Bub2/Bfa1/Tem1 at spindle poles, indicating that GTP hydrolysis is essential for asymmetry. Constitutive tethering of Bub2 or Bfa1 to both spindle poles impairs SPOC response but does not impair mitotic exit. Rather, it facilitates mitotic exit of MEN mutants, likely by increasing the residence time of Tem1 at spindle poles where it gets active. Surprisingly, all mutant or chimeric proteins leading to symmetrical localization of Bub2/Bfa1/Tem1 lead to increased symmetry at spindle poles of the Kar9 protein that mediates spindle positioning and cause spindle misalignment. Thus, asymmetry of the Bub2/Bfa1/Tem1 complex is crucial to control Kar9 distribution and spindle positioning during mitosis.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Public Library of Science (PLoS)
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/4.0/
dc.title
Asymmetry of the Budding Yeast Tem1 GTPase at Spindle Poles Is Required for Spindle Positioning But Not for Mitotic Exit
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 4.0 International
ethz.journal.title
PLoS Genetics
ethz.journal.volume
11
en_US
ethz.journal.issue
2
en_US
ethz.journal.abbreviated
PLoS Genet
ethz.pages.start
e1004938
en_US
ethz.size
29 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.identifier.wos
ethz.identifier.scopus
ethz.identifier.nebis
005410305
ethz.publication.place
San Francisco, CA
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03532 - Barral, Yves / Barral, Yves
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02030 - Dep. Biologie / Dep. of Biology::02517 - Institut für Biochemie / Institute of Biochemistry (IBC)::03532 - Barral, Yves / Barral, Yves
ethz.date.deposited
2017-06-11T16:48:41Z
ethz.source
ECIT
ethz.identifier.importid
imp593653105b9cf91718
ethz.ecitpid
pub:155985
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-12T23:20:06Z
ethz.rosetta.lastUpdated
2018-11-05T10:38:29Z
ethz.rosetta.versionExported
true
ethz.COinS
ctx_ver=Z39.88-2004&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.atitle=Asymmetry%20of%20the%20Budding%20Yeast%20Tem1%20GTPase%20at%20Spindle%20Poles%20Is%20Required%20for%20Spindle%20Positioning%20But%20Not%20for%20Mitotic%20Exit&rft.jtitle=PLoS%20Genetics&rft.date=2015-02-06&rft.volume=11&rft.issue=2&rft.spage=e1004938&rft.issn=1553-7390&1553-7404&rft.au=Scarfone,%20Ilaria&Venturetti,%20Marianna&Hotz,%20Manuel&Lengefeld,%20Jette&Barral,%20Yves&rft.genre=article&rft_id=info:doi/10.1371/journal.pgen.1004938&
 Search print copy at ETH Library

Files in this item

Thumbnail

Publication type

Show simple item record