B- and T-cell acute lymphoblastic leukemias evade chemotherapy at distinct sites in the bone marrow


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Date

2023-05

Publication Type

Journal Article

ETH Bibliography

yes

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Data

Abstract

Persistence of residual disease after induction chemotherapy is a strong predictor of relapse in acute lymphoblastic leukemia (ALL). The bone marrow microenvironment may support escape from treatment. Using three-dimensional fluorescence imaging of ten primary ALL xenografts we identified sites of predilection in the bone marrow for resistance to induction with dexamethasone, vincristine and doxorubicin. We detected B-cell precursor ALL cells predominantly in the perisinusoidal space at early engraftment and after chemotherapy. The spatial distribution of T-ALL cells was more widespread with contacts to endosteum, nestin+ pericytes and sinusoids. Dispersion of T-ALL cells in the bone marrow increased under chemotherapeutic pressure. A subset of slowly dividing ALL cells was transiently detected upon short-term chemotherapy, but not at residual disease after chemotherapy, challenging the notion that ALL cells escape treatment by direct induction of a dormant state in the niche. These lineage-dependent differences point to niche interactions that may be more specifically exploitable to improve treatment.

Publication status

published

Editor

Book title

Journal / series

Volume

108 (5)

Pages / Article No.

1244 - 1258

Publisher

Fondazione Ferrata Storti

Event

Edition / version

Methods

Software

Geographic location

Date collected

Date created

Subject

Organisational unit

03992 - Schroeder, Timm / Schroeder, Timm check_circle

Notes

Funding

186271 - Elucidating the human mesenchymal bone marrow stromal hierarchy in health and disease (SNF)

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