Multiomic profiling of the acute stress response in the mouse hippocampus
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Date
2022-04-05
Publication Type
Journal Article
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yes
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Abstract
The acute stress response mobilizes energy to meet situational demands and re-establish homeostasis. However, the underlying molecular cascades are unclear. Here, we use a brief swim exposure to trigger an acute stress response in mice, which transiently increases anxiety, without leading to lasting maladaptive changes. Using multiomic profiling, such as proteomics, phospho-proteomics, bulk mRNA-, single-nuclei mRNA-, small RNA-, and TRAP-sequencing, we characterize the acute stress-induced molecular events in the mouse hippocampus over time. Our results show the complexity and specificity of the response to acute stress, highlighting both the widespread changes in protein phosphorylation and gene transcription, and tightly regulated protein translation. The observed molecular events resolve efficiently within four hours after initiation of stress. We include an interactive app to explore the data, providing a molecular resource that can help us understand how acute stress impacts brain function in response to stress.
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published
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Journal / series
Volume
13
Pages / Article No.
1824
Publisher
Nature
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Organisational unit
09499 - Bohacek, Johannes / Bohacek, Johannes
09783 - Gapp, Katharina / Gapp, Katharina
09658 - von Meyenn, Ferdinand / von Meyenn, Ferdinand
03742 - Zeilhofer, Hanns U. / Zeilhofer, Hanns U.
02207 - Functional Genomics Center Zurich / Functional Genomics Center Zurich
Notes
Funding
ETH-20 19-1 - A cross‐disciplinary, data‐driven approach to predict stress resilience from large‐scale behavioral, molecular and neural activity data (ETHZ)
172889 - Dissecting stress-induced molecular changes in circuits underlying anxiety (SNF)
ETH-25 20-2 - Actionable transcriptional networks underlying brain cells’ response to stimuli (ETHZ)
803491 - Elucidating the molecular mechanisms underlying brite adipocyte specification and activation (EC)
172889 - Dissecting stress-induced molecular changes in circuits underlying anxiety (SNF)
ETH-25 20-2 - Actionable transcriptional networks underlying brain cells’ response to stimuli (ETHZ)
803491 - Elucidating the molecular mechanisms underlying brite adipocyte specification and activation (EC)