Journal: Histochemistry and Cell Biology

Abbreviation

Histochem Cell Biol

Publisher

Springer

Journal Volumes

ISSN

0948-6143
1432-119X

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Publications 1 - 10 of 14
  • Extra cellular matrix remodelling after heterotopic rat heart transplantation
    Item type: Journal Article
    Franz, Marcus; Gruen, K.; Richter, P.; et al. (2010)
    Histochemistry and Cell Biology
  • A comparative analysis of oncofetal fibronectin and tenascin-C incorporation in tumour vessels using human recombinant SIP format antibodies
    Item type: Journal Article
    Berndt, Alexander; Köllner, Robert; Richter, Petra; et al. (2010)
    Histochemistry and Cell Biology
  • Widefield fluorescence microscopy with extended resolution
    Item type: Review Article
    Stemmer, Andreas; Beck, Markus; Fiolka, Reto (2008)
    Histochemistry and Cell Biology
  • Differential basolateral–apical distribution of scavenger receptor, class B, type I in cultured cells and the liver
    Item type: Journal Article
    Fruhwürth, Stefanie; Kovacs, Werner; Bittman, Robert; et al. (2014)
    Histochemistry and Cell Biology
    The high-density lipoprotein (HDL) receptor, scavenger receptor class B, type I (SR-BI), mediates selective cholesteryl ester uptake into the liver, which finally results in cholesterol secretion into the bile. Despite several reports, the distribution of hepatic SR-BI between the sinusoidal and canalicular membranes is still under debate. We present immunohistological data using specific markers showing that the bulk of SR-BI is present in sinusoidal membranes and, to a lesser extent, in canalicular membranes in murine and human liver sections. In addition, SR-BI was detected in preparations of rat liver canalicular membranes. We also compared the in vivo findings to HepG2 cells, a widely used in vitro hepatocyte model. Interestingly, SR-BI was enriched in bile canalicular-like (BC-like) structures in polarized HepG2 cells, which were cultivated either conventionally to form a monolayer or in Matrigel to form three-dimensional structures. Fluorescently labeled HDL was transported into close proximity of BC-like structures, whereas HDL labeled with the fluorescent cholesterol analog BODIPY-cholesterol was clearly detected within these structures. Importantly, similarly to human and mouse liver, SR-BI was localized in basolateral membranes in three-dimensional liver microtissues from primary human liver cells. Our results demonstrate that SR-BI is highly enriched in sinusoidal membranes and is also found in canalicular membranes. There was no significant basolateral–apical redistribution of hepatic SR-BI in fasting and refeeding experiments in mice. Furthermore, in vitro studies in polarized HepG2 cells showed explicit differences as SR-BI was highly enriched in BC-like structures. These structures are, however, functional and accumulated HDL-derived cholesterol. Thus, biological relevant model systems should be employed when investigating SR-BI distribution in vitro.
  • Localization of the pre-squalene segment of the isoprenoid biosynthetic pathway in mammalian peroxisomes
    Item type: Journal Article
    Kovacs, Werner J.; Tape, Khanichi N.; Shackelford, Janis E.; et al. (2007)
    Histochemistry and Cell Biology
  • Differential vascular expression and regulation of oncofetal tenascin-C and fibronectin variants in renal cell carcinoma (RCC)
    Item type: Journal Article
    Galler, Kerstin; Junker, Kerstin; Franz, Marcus; et al. (2012)
    Histochemistry and Cell Biology
  • Micrometer-resolution X-ray tomographic full-volume reconstruction of an intact post-mortem juvenile rat lung
    Item type: Journal Article
    Borisova, Elena; Lovric, Goran; Miettinen, Arttu; et al. (2021)
    Histochemistry and Cell Biology
    In this article, we present an X-ray tomographic imaging method that is well suited for pulmonary disease studies in animal models to resolve the full pathway from gas intake to gas exchange. Current state-of-the-art synchrotron-based tomographic phase-contrast imaging methods allow for three-dimensional microscopic imaging data to be acquired non-destructively in scan times of the order of seconds with good soft tissue contrast. However, when studying multi-scale hierarchically structured objects, such as the mammalian lung, the overall sample size typically exceeds the field of view illuminated by the X-rays in a single scan and the necessity for achieving a high spatial resolution conflicts with the need to image the whole sample. Several image stitching and calibration techniques to achieve extended high-resolution fields of view have been reported, but those approaches tend to fail when imaging non-stable samples, thus precluding tomographic measurements of large biological samples, which are prone to degradation and motion during extended scan times. In this work, we demonstrate a full-volume three-dimensional reconstruction of an intact rat lung under immediate post-mortem conditions and at an isotropic voxel size of (2.75 µm)3. We present the methodology for collecting multiple local tomographies with 360° extended field of view scans followed by locally non-rigid volumetric stitching. Applied to the lung, it allows to resolve the entire pulmonary structure from the trachea down to the parenchyma in a single dataset.
  • Feasibility and safety of synchrotron-based X-ray phase contrast imaging as a technique complementary to histopathology analysis
    Item type: Journal Article
    Li, Kan Yan Chloe; Dejea, Hector; De Winne, Koen; et al. (2023)
    Histochemistry and Cell Biology
    X-ray phase contrast imaging (X-PCI) is a powerful technique for high-resolution, three-dimensional imaging of soft tissue samples in a non-destructive manner. In this technical report, we assess the quality of standard histopathological techniques performed on formalin-fixed, paraffin-embedded (FFPE) human tissue samples that have been irradiated with different doses of X-rays in the context of an X-PCI experiment. The data from this study demonstrate that routine histochemical and immunohistochemical staining quality as well as DNA and RNA analyses are not affected by previous X-PCI on human FFPE samples. From these data we conclude it is feasible and acceptable to perform X-PCI on FFPE human biopsies.
  • OME-Zarr: a cloud-optimized bioimaging file format with international community support
    Item type: Journal Article
    Moore, Josh; Basurto-Lozada, Daniela; Besson, Sébastien; et al. (2023)
    Histochemistry and Cell Biology
    A growing community is constructing a next-generation file format (NGFF) for bioimaging to overcome problems of scalability and heterogeneity. Organized by the Open Microscopy Environment (OME), individuals and institutes across diverse modalities facing these problems have designed a format specification process (OME-NGFF) to address these needs. This paper brings together a wide range of those community members to describe the cloud-optimized format itself-OME-Zarr-along with tools and data resources available today to increase FAIR access and remove barriers in the scientific process. The current momentum offers an opportunity to unify a key component of the bioimaging domain-the file format that underlies so many personal, institutional, and global data management and analysis tasks.
  • Utrophin is lacking at the neuromuscular junctions in the extraocular muscles of normal cat
    Item type: Journal Article
    Assadi, Maziar; Müntener, Markus (2005)
    Histochemistry and Cell Biology
Publications 1 - 10 of 14