An open-source computational and data resource to analyze digital maps of immunopeptidomes


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Date

2015-07

Publication Type

Journal Article

ETH Bibliography

yes

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Abstract

We present a novel mass spectrometry-based high-throughput workflow and an open-source computational and data resource to reproducibly identify and quantify HLA-associated peptides. Collectively, the resources support the generation of HLA allele-specific peptide assay libraries consisting of consensus fragment ion spectra, and the analysis of quantitative digital maps of HLA peptidomes generated from a range of biological sources by SWATH mass spectrometry (MS). This study represents the first community-based effort to develop a robust platform for the reproducible and quantitative measurement of the entire repertoire of peptides presented by HLA molecules, an essential step towards the design of efficient immunotherapies.

Publication status

published

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Journal / series

Volume

4

Pages / Article No.

Publisher

eLife Sciences Publications

Event

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Methods

Software

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Organisational unit

03663 - Aebersold, Rudolf (emeritus) / Aebersold, Rudolf (emeritus) check_circle

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