Force-Controlled Formation of Dynamic Nanopores for Single-Biomolecule Sensing and Single-Cell Secretomics
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Date
2020-10-27
Publication Type
Journal Article
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Abstract
Nanopore sensing of single nucleotides has emerged as a promising single-molecule technology for DNA sequencing and proteomics. Despite the conceptual simplicity of nanopores, adoption of this technology for practical applications has been limited by a lack of pore size adjustability and an inability to perform long-term recordings in complex solutions. Here we introduce a method for fast and precise on-demand formation of a nanopore with controllable size between 2 and 20 nm through force-controlled adjustment of the nanospace formed between the opening of a microfluidic device (made of silicon nitride) and a soft polymeric substrate. The introduced nanopore system enables stable measurements at arbitrary locations. By accurately positioning the nanopore in the proximity of single neurons and continuously recording single-molecule translations over several hours, we have demonstrated this is a powerful approach for single-cell proteomics and secretomics.
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published
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Journal / series
Volume
14 (10)
Pages / Article No.
12993 - 13003
Publisher
American Chemical Society
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Date collected
Date created
Subject
dynamic nanopores; single-cell proteomics; biomolecular sensors; AFM-integrated nanopores; nanoconfinement of biomolecules
Organisational unit
03640 - Vogel, Viola / Vogel, Viola
09831 - Aramesh, Morteza / Aramesh, Morteza
Notes
Funding
174217 - Single Entities at High Magnification: Mapping, Measuring and Manipulating Nanoparticles (SNF)