Glucose stress causes mRNA retention in nuclear Nab2 condensates
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Date
2024-01-23
Publication Type
Journal Article
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yes
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Abstract
Nuclear mRNA export via nuclear pore complexes is an essential step in eukaryotic gene expression. Although factors involved in mRNA transport have been characterized, a comprehensive mechanistic understanding of this process and its regulation is lacking. Here, we use single-RNA imaging in yeast to show that cells use mRNA retention to control mRNA export during stress. We demonstrate that, upon glucose withdrawal, the essential RNA-binding factor Nab2 forms RNA-dependent condensate-like structures in the nucleus. This co-incides with a reduced abundance of the DEAD-box ATPase Dbp5 at the nuclear pore. Depleting Dbp5, and consequently blocking mRNA export, is necessary and sufficient to trigger Nab2 condensation. The state of Nab2 condensation influences the extent of nuclear mRNA accumulation and can be recapitulated in vitro, where Nab2 forms RNA-dependent liquid droplets. We hypothesize that cells use condensation to regulate mRNA export and control gene expression during stress.
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published
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Journal / series
Volume
43 (1)
Pages / Article No.
113593
Publisher
Cell Press
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Date collected
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Subject
RNA; mRNA; condensation; Nab2; DEAD-box ATPases Dbp5; glucose stress
Organisational unit
09464 - Weis, Karsten / Weis, Karsten
Notes
Funding
179275 - Structure and Function of the Nuclear Pore Complex (SNF)
193740 - CRSII5_193740 / 1 (SNF)
193740 - CRSII5_193740 / 1 (SNF)