A device for single leaf labelling with CO2 isotopes to study carbon allocation and partitioning in Arabidopsis thaliana
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Date
2013-11
Publication Type
Journal Article
ETH Bibliography
yes
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Abstract
Background
Plant biomass consists primarily of carbohydrates derived from photosynthesis. Monitoring the assimilation of carbon via the Calvin-Benson cycle and its subsequent utilisation is fundamental to understanding plant growth. The use of stable and radioactive carbon isotopes, supplied to plants as CO2, allows the measurement of fluxes through the intermediates of primary photosynthetic metabolism, long-distance transport of sugars in the vasculature, and the synthesis of structural and storage components.
Results
Here we describe the design of a system for supplying isotopically labelled CO2 to single leaves of Arabidopsis thaliana. We demonstrate that the system works well using short pulses of 14CO2 and that it can be used to produce robust qualitative and quantitative data about carbon export from source leaves to the sink tissues, such as the developing leaves and the roots. Time course experiments show the dynamics of carbon partitioning between storage as starch, local production of biomass, and export of carbon to sink tissues.
Conclusion
This isotope labelling method is relatively simple to establish and inexpensive to perform. Our use of 14CO2 helps establish the temporal and spatial allocation of assimilated carbon during plant growth, delivering data complementary to those obtained in recent studies using 13CO2 and MS-based metabolomics techniques. However, we emphasise that this labelling device could also be used effectively in combination with 13CO2 and MS-based techniques.
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Publication status
published
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Book title
Journal / series
Volume
9
Pages / Article No.
45
Publisher
BioMed Central
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Arabidopsis; Photosynthesis; Carbohydrate metabolism; Assimilate partitioning; Phloem transport; Isotope labelling; Sink-source relationships
Organisational unit
03707 - Zeeman, Samuel C. / Zeeman, Samuel C.