Automated platform for yeast cultivation and analysis of secreted phytase in nanoliter droplet arrays
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Author / Producer
Date
2017
Publication Type
Other Conference Item
ETH Bibliography
yes
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Abstract
We present a method for cultivation of yeast cells and analysis of secreted enzymes in nanoliter (nL) droplets. We combined droplet microfluidics with a spotting platform on top of a fluorescence microscope. Yeast cells are encapsulated in nanoliter volumes using a T-junction and transferred via capillary onto a glass plate with currently up to 10000 hydrophilic spots. Droplets on the plate are post-processed by adding further assay compounds and can be analysed by fluorescence microscopy and mass spectrometry.
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Publication status
published
External links
Editor
Book title
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2017)
Journal / series
Volume
Pages / Article No.
201 - 202
Publisher
Chemical and Biological Microsystems Society
Event
21st International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2017)
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Droplet microfluidics; Single-cell analysis; Mass spectrometry; Yeast; Phytase
Organisational unit
03807 - Dittrich, Petra / Dittrich, Petra
02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.
Notes
Extended abstract. Conference lecture on October 25, 2017.
Funding
324247 - Modeling the Emergence of Social Complexity and Order: How Individual and Societal Complexity Co-Evolve (EC)