A lysyl oxidase-responsive collagen peptide illuminates collagen remodeling in wound healing
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Date
2024-04
Publication Type
Journal Article
ETH Bibliography
yes
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Abstract
Tissue repair and fibrosis involve the dynamic remodeling of collagen, and accurate detection of these sites is of utmost importance. Here, we use a collagen peptide sensor (1) to visualize collagen formation and remodeling during wound healing in mice and humans. We show that the probe binds selectively to sites of collagen formation and remodeling at different stages of healing. Compared to conventional methods, the peptide sensor localizes preferentially to areas of collagen synthesis and remodeling at the wound edge and not in matured fibrillar collagen. We also demonstrate its applicability for in vivo wound imaging and for discerning differential remodeling in wounds of transgenic mice with altered collagen dynamics. Our findings show the value of 1 as a diagnostic tool to rapidly identify the sites of matrix remodeling in tissue sections, which will aid in the conception of new therapeutic strategies for fibrotic disorders and defective tissue repair.
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Publication status
published
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Editor
Book title
Journal / series
Volume
128
Pages / Article No.
11 - 20
Publisher
Elsevier
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Lysyl oxidase; Collagen; Wound healing; Tissue remodeling; Fibrosis
Organisational unit
03520 - Werner, Sabine / Werner, Sabine
03940 - Wennemers, Helma / Wennemers, Helma
Notes
Funding
209202 - Chemical Tools for Monitoring and Targeting Collagen Cross-linking (SNF)
212212 - Control of skin inflammation by fibroblast growth factor signaling in keratinocytes (SNF)
212212 - Control of skin inflammation by fibroblast growth factor signaling in keratinocytes (SNF)