Lifespan effects in male UM-HET3 mice treated with sodium thiosulfate, 16-hydroxyestriol, and late-start canagliflozin


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Date

2024-10

Publication Type

Journal Article

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Abstract

Genetically heterogeneous UM-HET3 mice born in 2020 were used to test possible lifespan effects of alpha-ketoglutarate (AKG), 2,4-dinitrophenol (DNP), hydralazine (HYD), nebivolol (NEBI), 16 alpha-hydroxyestriol (OH_Est), and sodium thiosulfate (THIO), and to evaluate the effects of canagliflozin (Cana) when started at 16 months of age. OH_Est produced a 15% increase (p = 0.0001) in median lifespan in males but led to a significant (7%) decline in female lifespan. Cana, started at 16 months, also led to a significant increase (14%, p = 0.004) in males and a significant decline (6%, p = 0.03) in females. Cana given to mice at 6 months led, as in our previous study, to an increase in male lifespan without any change in female lifespan, suggesting that this agent may lead to female-specific late-life harm. We found that blood levels of Cana were approximately 20-fold higher in aged females than in young males, suggesting a possible mechanism for the sex-specific disparities in its effects. NEBI was also found to produce a female-specific decline (4%, p = 0.03) in lifespan. None of the other tested drugs provided a lifespan benefit in either sex. These data bring to 7 the list of ITP-tested drugs that induce at least a 10% lifespan increase in one or both sexes, add a fourth drug with demonstrated mid-life benefits on lifespan, and provide a testable hypothesis that might explain the sexual dimorphism in lifespan effects of the SGLT2 inhibitor Cana.

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published

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Volume

46 (5)

Pages / Article No.

4657 - 4670

Publisher

Springer

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Subject

Alpha-ketoglutarate; SGLT2 inhibitor Canagliflozin; Lifespan

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Notes

A Correction to this article was published on 19 June 2025: https://link.springer.com/article/10.1007/s11357-025-01752-0. There are five places in the paper where a drug is mistakenly referred to as “16-hydroxyestriol.” The correct name of this drug is “16-hydroxyestradiol.”

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Is referenced by: 10.1007/s11357-024-01176-2Is referenced by: 10.1007/s11357-025-01752-0