A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells
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Author / Producer
Date
2015
Publication Type
Book Chapter
ETH Bibliography
yes
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Abstract
Human embryonic stem cells hold great promise for future biomedical applications such as disease modeling and regenerative medicine. However, these cells are notoriously difficult to culture and are refractory to common means of genetic manipulation, thereby limiting their range of applications. In this protocol, we present an easy and robust method of gene repression in human embryonic stem cells using lipofection of small interfering RNA (siRNA).
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Publication status
published
Editor
Book title
Embryonic Stem Cell Protocols
Journal / series
Volume
1341
Pages / Article No.
369 - 376
Publisher
Humana Press
Event
Edition / version
Third edition
Methods
Software
Geographic location
Date collected
Date created
Subject
Transfection; Human embryonic stem cells; Lipofection; siRNA mediated-knockdown; Gene silencing
Organisational unit
03978 - Wutz, Anton / Wutz, Anton