Marc Sulliger


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Sulliger

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Marc

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0000-0002-2706-3912

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Publications 1 - 3 of 3
  • Hyperspectral Imaging for High Throughput Optical Spectroscopy of pL Droplets
    Item type: Journal Article
    Sulliger, Marc; Ortega Arroyo, Jaime; Quidant, Romain (2025)
    Analytical Chemistry
    Droplet-based microfluidics is a powerful tool for high-throughput analysis of liquid samples with significant applications in biomedicine and biochemistry. Nevertheless, extracting content-rich information from single picolitre-sized droplets at high throughputs remains challenging due to the weak signals associated with these small volumes. Overcoming this limitation would be transformative for fields that rely on high-throughput screening, enabling broader multiparametric analysis. Here we present an integrated optofluidic platform that addresses this critical point by combining advanced hyperspectral imaging with self-referencing and measurement automation. With this approach our platform achieves high temporal and spectral resolution with shot-noise limited performance, allowing for the label-free interrogation of single droplet contents. To demonstrate the platform's capabilities, we first exploit its high temporal and spectral resolution to study rapid dynamic changes in the composition of a heterogeneous population of nanoparticles. Second, leveraging the platform's shot-noise limited performance and using a model DNA-AuNP sensor, we detect target DNA sequences down to 250 pM, thereby showcasing the platform's compatibility with demanding sensing applications. Finally, through measurement automation, we demonstrate multiplexed sample monitoring over hours. These findings show that our optofluidic platform not only helps to close the current gap in high-throughput droplet analysis, but also significantly advances the potential for content-rich characterization, ultimately enhancing the scope and effectiveness of high-throughput screening methods.
  • Integrated Optofluidic Platform for High Throughput Optical Spectroscopy in Picolitre Droplets
    Item type: Doctoral Thesis
    Sulliger, Marc (2025)
    Droplet microfluidics is a powerful tool for high throughput analysis of liquid samples with significant potential in many disciplines, including biomedicine and biochemistry. Nevertheless, extracting content-rich information from single picolitre-sized droplets at high throughputs remains challenging due to the weak signals associated with these small volumes. This thesis addresses that bottleneck by building an integrated optofluidic platform, which combines droplet microfluidics with advanced optical readout and data analysis techniques. For microfluidics, the platform leverages state-of-the-art polydimethylsiloxane chips with integrated valves to enable precise control over droplet formation and fluid handling. For optical detection, a hyperspectral imaging approach enables broadband spectral data acquisition, efficiently recording hundreds of spectra in a single shot over a spatially extended field of view. Finally, to guarantee accurate and stable measurements over long durations and spatial ranges, computational image analysis and self-referencing steps are applied. The platform’s capabilities for high temporal resolution and biosensing were demonstrated through absorbance spectroscopy in reaction monitoring and the detection of short DNA sequences. Furthermore, using fluorescence spectroscopy, the platform was employed to study the structure switching behavior of three serotonin aptamer variations in a multiparametric way, providing valuable insights for data-driven aptamer engineering. In summary, this work presents an integrated optofluidic platform that unlocks the full analytical potential of droplet-based high throughput screening by providing content-rich information at the level of individual picolitre-sized droplets.
  • Three-dimensional optofluidic control using reconfigurable thermal barriers
    Item type: Journal Article
    Schmidt, Falko; González-Gómez, Carlos David; Sulliger, Marc; et al. (2025)
    Nature Photonics
    Microfluidics allows for the precise control of small sample volumes through spatial confinement and exact routing of fluids. Usually, this is achieved by physical barriers. However, the rigidity of these barriers limits flexibility in certain applications. We introduce an optofluidic approach that leverages structured light and photothermal conversion to create dynamic, reconfigurable fluidic boundaries that can be easily integrated in existing setups. This system enables the controlled manipulation of fluids and particles by generating adjustable three-dimensional thermal landscapes. We demonstrate that our reconfigurable approach replicates the functions of traditional barriers and allows real-time adjustments for tasks such as individual particle steering and size-based sorting in heterogeneous mixtures. These results highlight the potential for adaptive and multifunctional microfluidic systems in applications such as chemical synthesis, lab-on-chip devices and microbiology.
Publications 1 - 3 of 3