Journal: Metallomics

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Publisher

Royal Society of Chemistry

Journal Volumes

ISSN

1756-5901
1756-591X

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2015 - 201922020 - 20233
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Publications 1 - 5 of 5
  • Coupling metal stable isotope compositions and X-ray absorption spectroscopy to study metal pathways in soil-plant systems: a mini review
    Item type: Review Article
    Aucour, Anne Marie; Sarret, Géraldine; Blommaert, Hester; et al. (2023)
    Metallomics
    Excess and limited trace metal contents in soils and plants can limit crop yields and pose a risk for the environment and human health. This mini-review reports on the emerging approach of combining X-ray absorption spectroscopy (XAS) with isotope analyses to improve the understanding of metal speciation and dynamics in soil-plant systems. In soils and their components, shifts in isotope compositions could be in some cases linked to changing metal speciation and thereby provide information on processes that control the phytoavailability of metals. In plants, the XAS-isotope approach has potential to improve the understanding of how complex interactions of metal speciation, redox processes, and membrane transport control metal uptake and translocation to edible plant parts. Yet, the XAS-isotope approach proves to be in a rather exploratory phase, and many research gaps remain. Such limitations can be overcome by methodological improvements and combining the approach with molecular biology and modelling approaches.
  • Impact of naturally occurring serine/cysteine variations on the structure and function of Pseudomonas metallothioneins
    Item type: Journal Article
    Habjanič, Jelena; Chesnov, Serge; Zerbe, Oliver; et al. (2020)
    Metallomics
    Metallothioneins (MTs), small cysteine-rich metal-binding proteins, support the viability of organisms under normal physiological conditions and help them to respond to different environmental stressors. Upon metal coordination (e.g. ZnII, CdII, CuI) they form characteristic polynuclear metal–thiolate clusters that are known for their high thermodynamic stability and kinetic lability. However, despite numerous studies, it is still not understood how MTs modulate their metal-binding properties. Pseudomonas MTs are an emerging subclass of bacterial MTs, distinct for their high number of His residues and for several unique features such as an intrinsically disordered long C-terminal tail and multiple variations in the number and nature of coordinating amino acids. These variations might provide the bacteria with a functional advantage derived from evolutionary adaptation to heterogeneous environments. Nearly 90% of the known Pseudomonas MT sequences feature a central YC[C with combining low line]xxC motif, that is altered to YC[S with combining low line]xxC in the rest. We demonstrate that the additional Cys residue serves as a coordinating ligand without influencing the metal-binding capacity, the overall metal-binding stability or the structure. However, the additional ligand changes intra-cluster dynamics and, as a consequence, modulates metal transfer reactions that could be functionally advantageous in vivo.
  • Dissolved cerium contributes to uptake of Ce in the presence of differently sized CeO2-nanoparticles by three crop plants
    Item type: Journal Article
    Schwabe, Franziska; Tanner, Simon; Schulin, R.; et al. (2015)
    Metallomics
  • Longitudinal biometal accumulation and Ca isotope composition of the Göttingen minipig brain
    Item type: Journal Article
    Mahan, Brandon; Antonelli, Michael A.; Burckel, Pierre; et al. (2020)
    Metallomics
    Biometals play a critical role in both the healthy and diseased brain's functioning. They accumulate in the normal aging brain, and are inherent to neurodegenerative disorders and their associated pathologies. A prominent example of this is the brain accumulation of metals such as Ca, Fe and Cu (and more ambiguously, Zn) associated with Alzheimer's disease (AD). The natural stable isotope compositions of such metals have also shown utility in constraining biological mechanisms, and in differentiating between healthy and diseased states, sometimes prior to conventional methods. Here we have detailed the distribution of the biologically relevant elements Mg, P, K, Ca, Fe, Cu and Zn in brain regions of Göttingen minipigs ranging in age from three months to nearly six years, including control animals and both a single- and double-transgenic model of AD (PS1, APP/PS1). Moreover, we have characterized the Ca isotope composition of the brain for the first time. Concentration data track rises in brain biometals with age, namely for Fe and Cu, as observed in the normal ageing brain and in AD, and biometal data point to increased soluble amyloid beta (Aβ) load prior to AD plaque identification via brain imaging. Calcium isotope results define the brain as the isotopically lightest permanent reservoir in the body, indicating that brain Ca dyshomeostasis may induce measurable isotopic disturbances in accessible downstream reservoirs such as biofluids.
  • A histidine-rich Pseudomonas metallothionein with a disordered tail displays higher binding capacity for cadmium than zinc
    Item type: Journal Article
    Habjanič, Jelena; Zerbe, Oliver; Freisinger, Eva (2018)
    Metallomics
Publications 1 - 5 of 5