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Quantitative analysis of multiple antibody-ligand interactions in a microchip using fluorescence polarization anisotropy


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Date

2013

Publication Type

Other Conference Item

ETH Bibliography

yes

Citations

Altmetric
METADATA ONLY

Data

Rights / License

Abstract

We present a microfluidic device that allows quantitative analysis of protein interactions with fluorescently labeled ligands. Dissociation constants (Kd), binding classes (m) as well as number of binding sites (n) can be determined in the same experiment, even if protein mixtures are present.

Publication status

published

External links

Editor

Book title

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)

Journal / series

Volume

Pages / Article No.

500 - 502

Publisher

Chemical and Biological Microsystems Society

Event

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2013)

Edition / version

Methods

Software

Geographic location

Date collected

Date created

Subject

Protein-ligand interaction; Dissociation constant; Binding studies

Organisational unit

03807 - Dittrich, Petra / Dittrich, Petra check_circle

Notes

Poster abstract. Poster presentation on October 28, 2013.

Funding

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