Notch signaling in the dynamics of perivascular stem cells and their niches
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Date
2021-10
Publication Type
Journal Article
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yes
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Abstract
The Notch signaling pathway is a fundamental regulator of cell fate determination in homeostasis and regeneration. In this work, we aimed to determine how Notch signaling mediates the interactions between perivascular stem cells and their niches in human dental mesenchymal tissues, both in homeostatic and regenerative conditions. By single cell RNA sequencing analysis, we showed that perivascular cells across the dental pulp and periodontal human tissues all express NOTCH3, and that these cells are important for the response to traumatic injuries in vivo in a transgenic mouse model. We further showed that the behavior of perivascular NOTCH3-expressing stem cells could be modulated by cellular and molecular cues deriving from their microenvironments. Taken together, the present studies, reinforced by single-cell analysis, reveal the pivotal importance of Notch signaling in the crosstalk between perivascular stem cells and their niches in tissue homeostasis and regeneration.
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published
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Journal / series
Volume
10 (10)
Pages / Article No.
1433 - 1445
Publisher
Wiley
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Edition / version
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Date collected
Date created
Subject
dental pulp stem cells; carious teeth; mesenchymal stem cells; microenvironment; Notch ligands; Notch signaling; Notch3; pericytes; periodontal stem cells; periodontium; perivascular niche; pulp; single-cell RNA sequencing; stem cell niches; stem cells; tooth
Organisational unit
09711 - Moor, Andreas / Moor, Andreas
Notes
Funding
197782 - Tooth-on-a-chip: development of a model for the simulation of human dental tissues (SNF)