Engineering of Therapeutic and Detoxifying Enzymes
METADATA ONLY
Loading...
Author / Producer
Date
2023-11-13
Publication Type
Review Article
ETH Bibliography
yes
Citations
Altmetric
METADATA ONLY
Data
Rights / License
Abstract
Therapeutic enzymes present excellent opportunities for the treatment of human disease, modulation of metabolic pathways and system detoxification. However, current use of enzyme therapy in the clinic is limited as naturally occurring enzymes are seldom optimal for such applications and require substantial improvement by protein engineering. Engineering strategies such as design and directed evolution that have been successfully implemented for industrial biocatalysis can significantly advance the field of therapeutic enzymes, leading to biocatalysts with new-to-nature therapeutic activities, high selectivity, and suitability for medical applications. This minireview highlights case studies of how state-of-the-art and emerging methods in protein engineering are explored for the generation of therapeutic enzymes and discusses gaps and future opportunities in the field of enzyme therapy.
Permanent link
Publication status
published
External links
Editor
Book title
Journal / series
Volume
62 (46)
Pages / Article No.
Publisher
Wiley-VCH
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Artificial Metalloenzymes; Base Editor; Directed Evolution; Enzyme Design; Enzyme Therapy
Organisational unit
Notes
Funding
846170 - Enzyme design and engineering by implementation of non-canonical amino acids in protein scaffolds (EC)