Journal: Biomedical Optics Express

Abbreviation

Biomed. opt. express

Publisher

OSA Publishing

Journal Volumes

ISSN

2156-7085

Description

Filters

2011 - 2019122020 - 20214
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Publications 1 - 10 of 16
  • Toward whole-brain in vivo optoacoustic angiography of rodents: modeling and experimental observations
    Item type: Journal Article
    Subochev, Pavel; Smolina, Ekaterina; Sergeeva, Ekaterina; et al. (2020)
    Biomedical Optics Express
  • Concurrent fluorescence and volumetric optoacoustic tomography of nanoagent perfusion and bio-distribution in solid tumors
    Item type: Journal Article
    Chen, Zhenyue; Dean-Ben, Xose Luis; Liu, Nian; et al. (2019)
    Biomedical Optics Express
  • POF-yarn weaves: Controlling the light outcoupling of wearable phototherapy devices
    Item type: Journal Article
    Quandt, Brit M.; Pfister, Marisa S.; Lübben, Jörn; et al. (2017)
    Biomedical Optics Express
  • Detection of cerebral tauopathy in P301L mice using high-resolution large-field multifocal illumination fluorescence microscopy
    Item type: Journal Article
    Ni, Ruiqing; Chen, Zhenyue; Gerez, Juan A.; et al. (2020)
    Biomedical Optics Express
    Current intravital microscopy techniques visualize tauopathy with high-resolution, but have a small field-of-view and depth-of-focus. Herein, we report a transcranial detection of tauopathy over the entire cortex of P301L tauopathy mice using large-field multifocal illumination (LMI) fluorescence microscopy technique and luminescent conjugated oligothiophenes. In vitro assays revealed that fluorescent ligand h-FTAA is optimal for in vivo tau imaging, which was confirmed by observing elevated probe retention in the cortex of P301L mice compared to non-transgenic littermates. Immunohistochemical staining further verified the specificity of h-FTAA to detect tauopathy in P301L mice. The new imaging platform can be leveraged in pre-clinical mechanistic studies of tau spreading and clearance as well as longitudinal monitoring of tau targeting therapeutics.
  • Compact ultrafast semiconductor disk laser
    Item type: Journal Article
    Aviles-Espinosa, Rodrigo; Filippidis, George; Hamilton, Craig; et al. (2011)
    Biomedical Optics Express
  • Noninvasive multimodal fluorescence and magnetic resonance imaging of whole-organ intervertebral discs
    Item type: Journal Article
    Ren, Wuwei; Cui, Shangbin; Alini, Mauro; et al. (2021)
    Biomedical Optics Express
    Low back pain (LBP) is a commonly experienced symptom posing a tremendous healthcare burden to individuals and society at large. The LBP pathology is strongly linked to degeneration of the intervertebral disc (IVD), calling for development of early-stage diagnostic tools for visualizing biomolecular changes in IVD. Multimodal measurements of fluorescence molecular tomography (FMT) and magnetic resonance imaging (MRI) were performed on IVD whole organ culture model using an in-house built FMT system and a high-field MRI scanner. The resulted multimodal images were systematically validated through epifluorescence imaging of the IVD sections at a microscopic level. Multiple image contrasts were exploited, including fluorescence distribution, anatomical map associated with T1-weighted MRI contrast, and water content related with T2 relaxation time. The developed multimodality imaging approach may thus serve as a new assessment tool for early diagnosis of IVD degeneration and longitudinal monitoring of IVD organ culture status using fluorescence markers.
  • Fast TIRF-SIM imaging of dynamic, low-fluorescent biological samples
    Item type: Journal Article
    Roth, Julian; Mehl, Johanna; Rohrbach, Alexander (2020)
    Biomedical Optics Express
    Fluorescence microscopy is the standard imaging technique to investigate the structures and dynamics of living cells. However, increasing the spatial resolution comes at the cost of temporal resolution and vice versa. In addition, the number of images that can be taken in sufficiently high quality is limited by fluorescence bleaching. Hence, super-resolved imaging at several Hertz of low fluorescent biological samples is still a big challenge and, especially in structured illumination microscopy (SIM), is often visible as imaging artifacts. In this paper, we present a TIRF-SIM system based on scan-mirrors and a Michelson interferometer, which generates images at 110 nm spatial resolution and up to 8 Hz temporal resolution. High resolution becomes possible by optimizing the illumination interference contrast, even for low fluorescent, moving samples. We provide a framework and guidelines on how the modulation contrast, which depends on laser coherence, polarization, beam displacement or sample movements, can be mapped over the entire field of view. In addition, we characterize the influence of the signal-to-noise ratio and the Wiener filtering on the quality of reconstructed SIM images, both in real and frequency space. Our results are supported by theoretical descriptions containing the parameters leading to image artifacts. This study aims to help microscopists to better understand and adjust optical parameters for structured illumination, thereby leading to more trustworthy measurements and analyses of biological dynamics. © 2020 Optical Society of America.
  • X-ray phase microtomography with a single grating for high-throughput investigations of biological tissue
    Item type: Journal Article
    Zdora, Marie-Christine; Vila-Comamala, Joan; Schulz, Georg; et al. (2017)
    Biomedical Optics Express
    The high-throughput 3D visualisation of biological specimens is essential for studying diseases and developmental disorders. It requires imaging methods that deliver high-contrast, high-resolution volumetric information at short sample preparation and acquisition times. Here we show that X-ray phase-contrast tomography using a single grating can provide a powerful alternative to commonly employed techniques, such as high-resolution episcopic microscopy (HREM). We present the phase tomography of a mouse embryo in paraffin obtained with an X-ray single-grating interferometer at I13-2 Beamline at Diamond Light Source and discuss the results in comparison with HREM measurements. The excellent contrast and quantitative density information achieved non-destructively and without staining using a simple, robust setup make X-ray single-grating interferometry an optimum candidate for high-throughput imaging of biological specimens as an alternative for existing methods like HREM.
  • Enhanced robustness digital holographic microscopy for demanding environment of space biology
    Item type: Journal Article
    Toy, M. Fatih; Richard, Stéphane; Kuhn, Jonas; et al. (2012)
    Biomedical Optics Express
  • Glucose sensing in human epidermis using mid-infrared photoacoustic detection
    Item type: Journal Article
    Kottmann, Jonas; Rey, Julien M.; Luginbühl, Joachim; et al. (2012)
    Biomedical Optics Express
Publications 1 - 10 of 16