Ilaria Incaviglia


Last Name

Incaviglia

First Name

Ilaria

ORCID

0000-0002-1982-2253

Organisational unit

03870 - Müller, Daniel J. / Müller, Daniel J.

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2021 - 20256
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Publications 1 - 6 of 6
  • Tailoring the Sensitivity of Microcantilevers To Monitor the Mass of Single Adherent Living Cells
    Item type: Journal Article
    Incaviglia, Ilaria; Herzog, Sophie; Fläschner, Gotthold Viktor; et al. (2023)
    Nano Letters
    Microcantilevers are widely employed as mass sensors for biological samples, from single molecules to single cells. However, the accurate mass quantification of living adherent cells is impaired by the microcantilever’s mass sensitivity and cell migration, both of which can lead to detect masses mismatching by ≫50%. Here, we design photothermally actuated microcantilevers to optimize the accuracy of cell mass measurements. By reducing the inertial mass of the microcantilever using a focused ion beam, we considerably increase its mass sensitivity, which is validated by finite element analysis and experimentally by gelatin microbeads. The improved microcantilevers allow us to instantly monitor at much improved accuracy the mass of both living HeLa cells and mouse fibroblasts adhering to different substrates. Finally, we show that the improved cantilever design favorably restricts cell migration and thus reduces the large measurement errors associated with this effect.
  • Optimizing the Sensitivity of Microcantilevers to Monitor the Mass of Single Adherent Living Cells
    Item type: Other Conference Item
    Incaviglia, Ilaria; Herzog, Sophie; Fläschner, Gotthold Viktor; et al. (2023)
    Biophysical Journal
  • Harmony in Motion: Unveiling Single Cell Dynamics with Simultaneous Mass and Stiffness Measurements
    Item type: Other Conference Item
    Incaviglia, Ilaria; Ammirati, Giulia; Krug, Tommy; et al. (2024)
    Nanoengineering for Mechanobiology 2024 Abstracts
  • Additively Manufactured Semi-Flexible Titanium Lattices as Hydrogel Reinforcement for Biomedical Implants
    Item type: Journal Article
    Tosoratti, Enrico; Incaviglia, Ilaria; Liashenko, Oleksii; et al. (2021)
    Advanced NanoBiomed Research
    Hydrogels are one of the most widespread biomaterials used in tissue engi- neering. However, they possess weak mechanical properties and are often unstable in load-bearing applications in vivo. A novel class of exible Ti–6Al–4V titanium alloy lattices manufactured using laser powder bed fusion (L-PBF) serves as a tunable reinforcement for hydrogels, providing them with additional mechanical stability and exibility, while ensuring biocompatibility. A study on the design parameters of the structural elements of the lattices is performed to evaluate their inuence on the mechanical properties of the structure. Mechanical testing of Ti–6Al–4V lattices shows a compressive modulus ranging from 38.9 to 895.5 kPa in the exible direction. In the other two directions, the lattices are designed to have minimal exibility. Lattices embedded in a 1% agarose hydrogel show a strain-rate-dependent, viscoelastic behavior given by the hydrogel component with the additional stiffness of the titanium lattice. Stress distribution upon lo ading is simulated using nite element analysis (FEA) and compared to experimental data using multiple regression statistical analysis. As a proof of concept, an intervertebral spinal disc implant is designed with mechanical properties matching the compressive moduli of the nucleus pulposus and anulus brosus reported in the literature.
  • Monitoring the mass, eigenfrequency, and quality factor of mammalian cells
    Item type: Journal Article
    Herzog, Sophie; Fläschner, Gotthold Viktor; Incaviglia, Ilaria; et al. (2024)
    Nature Communications
    The regulation of mass is essential for the development and homeostasis of cells and multicellular organisms. However, cell mass is also tightly linked to cell mechanical properties, which depend on the time scales at which they are measured and change drastically at the cellular eigenfrequency. So far, it has not been possible to determine cell mass and eigenfrequency together. Here, we introduce microcantilevers oscillating in the Ångström range to monitor both fundamental physical properties of the cell. If the oscillation frequency is far below the cellular eigenfrequency, all cell compartments follow the cantilever motion, and the cell mass measurements are accurate. Yet, if the oscillating frequency approaches or lies above the cellular eigenfrequency, the mechanical response of the cell changes, and not all cellular components can follow the cantilever motions in phase. This energy loss caused by mechanical damping within the cell is described by the quality factor. We use these observations to examine living cells across externally applied mechanical frequency ranges and to measure their total mass, eigenfrequency, and quality factor. The three parameters open the door to better understand the mechanobiology of the cell and stimulate biotechnological and medical innovations.
  • Nanotools to Simultaneously Measure Mass and Mechanical Properties of Cellular Systems
    Item type: Doctoral Thesis
    Incaviglia, Ilaria (2025)
Publications 1 - 6 of 6