Dicer, a new regulator of pluripotency exit and LINE-1 elements in mouse embryonic stem cells
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Date
2017-02
Publication Type
Journal Article
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yes
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Abstract
gene regulation network orchestrates processes ensuring the maintenance of cellular identity and genome integrity. Small RNAs generated by the RNAse III DICER have emerged as central players in this network. Moreover, deletion of Dicer in mice leads to early embryonic lethality. To better understand the underlying mechanisms leading to this phenotype, we generated Dicer-deficient mouse embryonic stem cells (mESCs). Their detailed characterization revealed an impaired differentiation potential, and incapacity to exit from the pluripotency state. We also observed a strong accumulation of LINE-1 (L1s) transcripts, which was translated at protein level and led to an increased L1s retrotransposition. Our findings reveal Dicer as a new essential player that sustains mESCs self-renewal and genome integrity by controlling L1s regulation.
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Publication status
published
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Journal / series
Volume
7 (2)
Pages / Article No.
204 - 220
Publisher
Wiley
Event
Edition / version
Methods
Software
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Date collected
Date created
Subject
Dicer; LINE-1 retrotransposition; mouse embryonic stem cells; transposable elements
Organisational unit
03983 - Ciaudo, C. (ehemalig) / Ciaudo, C. (former)
Notes
Funding
153220 - Nuclear role of the Argonaute proteins in mammalian embryonic stem cells (SNF)