Claire Guinat

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Guinat

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Claire

ORCID

0000-0002-8245-9290

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Publications 1 - 10 of 32

Unravelling direct and indirect contact patterns between duck farms in France and their association with the 2016–2017 epidemic of Highly Pathogenic Avian Influenza (H5N8)
Bauzile, Billy; Sicard, G.; Guinat, Claire; et al. (2022)
Preventive Veterinary Medicine
Live animal movements generate direct contacts (via the exchange of live animals) and indirect contacts (via the transit of transport vehicles) between farms, which can contribute to the spread of pathogens. However, most analyses focus solely on direct contacts and can therefore underestimate the contribution of live animal movements in the spread of infectious diseases. Here, we used French live duck movement data (2016–2018) from one of the largest transport companies to compare direct and indirect contact patterns between duck farms and evaluate how these patterns were associated with the French 2016–2017 epidemic of highly pathogenic avian influenza H5N8. A total number of 614 farms were included in the study, and two directed networks were generated: the animal introduction network (exchange of live ducks) and the transit network (transit of transport vehicles). Following descriptive analyses, these two networks were scrutinized in relation to farm infection status during the epidemic. Results showed that farms were substantially more connected in the transit network than in the animal introduction network and that the transit of transport vehicles generated more opportunities for transmission than the exchange of live animals. We also showed that animal introduction and transit networks’ statistics decreased substantially during the epidemic (January–March 2017) compared to non-epidemic periods (January–March 2016 and January–March 2018). We estimated a probability of 33.3 % that a farm exposed to the infection through either of the two live duck movement networks (i.e. that was in direct or indirect contact with a farm that was reported as infected in the following seven days) becomes infected within seven days after the contact. However, we also demonstrated that the level of exposure of farms by these two contact patterns was low, leading only to a handful of transmission events through these routes. As a consequence, we showed that live animal movement patterns are efficient transmission routes for HPAI but have been efficiently reduced to limit the spread during the French 2020–2021 epidemic. These results underpin the relevance of studying indirect contacts resulting from the movement of animals to understand their transmission potential and the importance of accounting for both routes when designing disease control strategies.
Survival of African Swine Fever Virus in Excretions from Pigs Experimentally Infected with the Georgia 2007/1 Isolate
Davies, Katherine; Goatley, Lynnette C.; Guinat, Claire; et al. (2017)
Transboundary and Emerging Diseases
African swine fever virus (ASFV) causes a lethal haemorrhagic disease of swine which can be transmitted through direct contact with infected animals and their excretions or indirect contact with contaminated fomites. The shedding of ASFV by infected pigs and the stability of ASFV in the environment will determine the extent of environmental contamination. The recent outbreaks of ASF in Europe make it essential to develop disease transmission models in order to design effective control strategies to prevent further spread of ASF. In this study, we assessed the shedding and stability of ASFV in faeces, urine and oral fluid from pigs infected with the Georgia 2007/1 ASFV isolate. The half‐life of infectious ASFV in faeces was found to range from 0.65 days when stored at 4°C to 0.29 days when stored at 37°C, while in urine it was found to range from 2.19 days (4°C) to 0.41 days (37°C). Based on these half‐lives and the estimated dose required for infection, faeces and urine would be estimated to remain infectious for 8.48 and 15.33 days at 4°C and 3.71 and 2.88 days at 37°C, respectively. The half‐life of ASFV DNA was 8 to 9 days in faeces and 2 to 3 days in oral fluid at all temperatures. In urine, the half‐life of ASFV DNA was found to be 32.54 days at 4°C decreasing to 19.48 days at 37°C. These results indicate that ASFV in excretions may be an important route of ASFV transmission.
Inferring within‐flock transmission dynamics of highly pathogenic avian influenza H5N8 virus in France, 2020
Vergne, Timothée; Gubbins, Simon; Guinat, Claire; et al. (2021)
Transboundary and Emerging Diseases
Following the emergence of highly pathogenic avian influenza (H5N8) in France in early December 2020, we used duck mortality data from the index farm to investigate within-flock transmission dynamics. A stochastic epidemic model was fitted to the daily mortality data and model parameters were estimated using an approximate Bayesian computation sequential Monte Carlo (ABC-SMC) algorithm. The model predicted that the first bird in the flock was infected 5 days (95% credible interval, CI: 3–6) prior to the day of suspicion and that the transmission rate was 4.1 new infections per day (95% CI: 2.8–5.8). On average, ducks became infectious 4.1 h (95% CI: 0.7–9.1) after infection and remained infectious for 4.3 days (95% CI: 2.8–5.7). The model also predicted that 34% (50% prediction interval: 8%–76%) of birds would already be infectious by the day of suspicion, emphasizing the substantial latent threat this virus could pose to other poultry farms and to neighbouring wild birds. This study illustrates how mechanistic models can help provide rapid relevant insights that contribute to the management of infectious disease outbreaks of farmed animals. These methods can be applied to future outbreaks and the resulting parameter estimates made available to veterinary services within a few hours.
Quantification and characterisation of commensal wild birds and their interactions with domestic ducks on a free-range farm in southwest France
Le Gall-Ladevèze, Chloé; Guinat, Claire; Fievet, Lucas; et al. (2022)
Scientific Reports
The role of commensal birds in the epidemiology of pathogens in poultry farms remains unclear. Our study aimed to identify potential key species for interactions with domestic ducks on one free-range duck farm in southwest France. Methods combined direct individual observations on duck outdoor foraging areas, network analysis, and general linear mixed models of abundances. Results showed a wide diversity of wild bird species visiting foraging areas, heavily dominated in frequency by White wagtails (Motacilla alba) and Sparrows (Passer domesticus and Passer montanus). These also were the only species seen entering duck premises or perching on drinkers in the presence of ducks. Moreover, White wagtails were the species most frequently observed on the ground and in close proximity to ducks. Network analysis suggested the role of White wagtails and Sparrows in linking ducks to other wild birds on the farm. The abundance of White wagtails was positively associated with open vegetation, with the presence of ducks and particularly in the afternoon, while the abundance of Sparrows was positively associated only with the fall-winter season. By precisely characterising interactions, the study was able to identify few wild bird species which should be prioritized in infectious investigations at the interface with poultry.
Effectiveness and practicality of control strategies for African swine fever: what do we really know?
Guinat, Claire; Vergne, Timothée; Jurado-Diaz, C.; et al. (2017)
Veterinary Record
African swine fever (ASF) is a major pig health problem, and the causative virus is moving closer to Western European regions where pig density is high. Stopping or slowing down the spread of ASF requires mitigation strategies that are both effective and practical. Based on the elicitation of ASF expert opinion, this study identified surveillance and intervention strategies for ASF that are perceived as the most effective by providing the best combination between effectiveness and practicality. Among the 20 surveillance strategies that were identified, passive surveillance of wild boar and syndromic surveillance of pig mortality were considered to be the most effective surveillance strategies for controlling ASF virus spread. Among the 22 intervention strategies that were identified, culling of all infected herds and movement bans for neighbouring herds were regarded as the most effective intervention strategies. Active surveillance and carcase removal in wild boar populations were rated as the most effective surveillance and intervention strategies, but were also considered to be the least practical, suggesting that more research is needed to develop more effective methods for controlling ASF in wild boar populations.
Role of Live-Duck Movement Networks in Transmission of Avian Influenza, France, 2016–2017
Guinat, Claire; Durand, Benoit; Vergne, Timothée; et al. (2020)
Emerging Infectious Diseases
The relative roles that movement and proximity networks play in the spread of highly pathogenic avian influenza (HPAI) viruses are often unknown during an epidemic, preventing effective control. We used network analysis to explore the devastating epidemic of HPAI A(H5N8) among poultry, in particular ducks, in France during 2016-2017 and to estimate the likely contribution of live-duck movements. Approximately 0.2% of live-duck movements could have been responsible for between-farm transmission events, mostly early during the epidemic. Results also suggest a transmission risk of 35.5% when an infected holding moves flocks to another holding within 14 days before detection. Finally, we found that densely connected groups of holdings with sparse connections between groups overlapped farmer organizations, which represents important knowledge for surveillance design. This study highlights the importance of movement bans in zones affected by HPAI and of understanding transmission routes to develop appropriate HPAI control strategies. © 2020 Centers for Disease Control and Prevention (CDC)
Backyard poultry flocks in France: A diversity of owners and biosecurity practices
Souvestre, Marie; Delpont, Mattias; Guinat, Claire; et al. (2021)
Preventive Veterinary Medicine
Over the past few years, the number of backyard poultry flocks has been increasing in France. A mandatory step to improve backyard poultry management is to assess health risks by characterizing the flocks and understanding the owners’ motivations for keeping poultry and their husbandry practices. A survey of backyard poultry owners was conducted in France to gather information about their motivations for owning poultry, flock characteristics, and breeding and biosecurity practices. The survey was completed by 1,160 owners. The major motivations for owning poultry flocks were egg consumption (93.3 %), recycling (72.4 %) and having pet animals (53.2 %). Most owners had already heard about avian influenza (96.7 %), but were less aware about other diseases such as Newcastle Disease (41.6 %), salmonellosis (79.1 %), or campylobacteriosis (18.6 %). Owners mainly kept only egg-layers (78.4 %), and the median size flock was five egg-layers. Owners gave eggs to their relatives, occasionally or regularly, in 86.6 % of the cases. Contacts with other family poultry owners were frequent (68.9 %) and biosecurity practices were poorly implemented: 50 % of owners did not wash their hands systematically after visiting the flock and more than 60 % of owners did not wear specific shoes. Drawing from the survey data, five profiles of family poultry flocks were identified with multiple correspondence analysis and hierarchical cluster analysis. The profiles, based on flock characteristics and owners’ practices and motivations, illustrate the heterogeneity of the backyard poultry sector: 1) urban poultry, 2) traditional poultry, 3) student poultry, 4) pet poultry and 5) hobby poultry. Urban poultry consisted of recently constituted (< 2 years old), small (< 3 birds) flocks of layers, and traditional poultry of older, medium-sized flocks belonging to retired and older people. These two profiles were characterized by limited contacts (direct or indirect) with other flocks and owners. Student poultry consisted of younger owners (<30 years old) with flocks over 5 years old. Pet poultry consisted of recently established, medium-size flocks of layers located in both rural or urban environments. Hobby poultry consisted of dedicated owners who breed and sell poultry and participate in exhibitions and poultry shows. Pet and hobby poultry profiles were characterized by greater knowledge of diseases and biosecurity practices, more bird movements, and reported more frequent clinical signs. The observation of different profiles can help target veterinary and public health education messages to prevent disease transmission in backyard poultry flocks in France.
Duck production systems and highly pathogenic avian influenza H5N8 in France, 2016–2017
Guinat, Claire; Artois, J.; Bronner, Anne; et al. (2019)
Scientific Reports
In winter 2016–2017, Highly Pathogenic Avian Influenza (HPAI) H5N8 virus spread across Europe, causing unprecedented epizootics. France was massively affected, resulting in the culling of over 6 million poultry. Boosted regression tree (BRT) models were used to quantify the association between spatial risk factors and HPAI H5N8 infection in poultry holdings and to generate predictive maps for HPAI infection. Three datasets were combined to build the model: a dataset of the reported outbreaks in poultry, a dataset of the poultry holdings where the virus has not been reported and a set of relevant spatial risk factors, including poultry production and trade, and water bird habitat. Results identified key associations between the ‘foie gras’ production systems and HPAI H5N8 risk of occurrence and indicate that strengthening surveillance of fattening duck production systems and making the transportation of fattening ducks more secure would be key priority options for HPAI prevention and control.
Risk of Anticoagulant Rodenticide Exposure for Mammals and Birds in Parc National Des Pyrénées, France
Lestrade, Marie; Vergne, Timothée; Guinat, Claire; et al. (2021)
Journal of Wildlife Diseases
The extensive use of anticoagulant rodenticides (ARs) to control rodent populations poses intoxication risks for wildlife: persistence of ARs in rodents can cause secondary exposure and poisoning of predators or scavengers. We investigated risk factors for wildlife exposure to ARs in the Parc National des Pyrénées (PNP), France, using a multivariable logistic regression analysis. A total of 157 liver samples were collected from carcasses of 10 mammal and three bird species found in the PNP between 2010 and 2018 and screened for presence of AR residues. First- and second-generation ARs were detected in more than 60% of red fox (Vulpes vulpes) and stone marten (Martes foina) samples and in around 40% of wild cat (Felis silvestris), European pine marten (Martes martes), American mink (Neovison vison), and Eurasian Buzzard (Buteo buteo) samples. Wildlife exposure to ARs was significantly associated with consumption of small mammals (odds ratio [OR]: 2.5, 95% confidence interval [CI]: 1.1–5.8) being collected in the Ossau valley (OR: 2.5, 95% CI: 1.1–6.1) and between 2013 and 2015 (OR: 4.8, 95% CI: 2.0–11.7). We identified wild species that could be targeted for risk-based surveillance program for AR secondary exposure and determined high risk areas in which alternative measures should be applied for rodent control.
Dynamics of African swine fever virus shedding and excretion in domestic pigs infected by intramuscular inoculation and contact transmission
Guinat, Claire; Reis, Ana L.; Netherton, Christopher L; et al. (2014)
Veterinary Research
African swine fever virus (ASFV) is a highly virulent swine pathogen that has spread across Eastern Europe since 2007 and for which there is no effective vaccine or treatment available. The dynamics of shedding and excretion is not well known for this currently circulating ASFV strain. Therefore, susceptible pigs were exposed to pigs intramuscularly infected with the Georgia 2007/1 ASFV strain to measure those dynamics through within- and between-pen transmission scenarios. Blood, oral, nasal and rectal fluid samples were tested for the presence of ASFV by virus titration (VT) and quantitative real-time polymerase chain reaction (qPCR). Serum was tested for the presence of ASFV-specific antibodies. Both intramuscular inoculation and contact transmission resulted in development of acute disease in all pigs although the experiments indicated that the pathogenesis of the disease might be different, depending on the route of infection. Infectious ASFV was first isolated in blood among the inoculated pigs by day 3, and then chronologically among the direct and indirect contact pigs, by day 10 and 13, respectively. Close to the onset of clinical signs, higher ASFV titres were found in blood compared with nasal and rectal fluid samples among all pigs. No infectious ASFV was isolated in oral fluid samples although ASFV genome copies were detected. Only one animal developed antibodies starting after 12 days post-inoculation. The results provide quantitative data on shedding and excretion of the Georgia 2007/1 ASFV strain among domestic pigs and suggest a limited potential of this isolate to cause persistent infection.

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