Dilara Perver


Last Name

Perver

First Name

Dilara

ORCID

0000-0001-9460-2667

Organisational unit

03640 - Vogel, Viola / Vogel, Viola

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2021 - 20254
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Publications 1 - 4 of 4
  • Extracellular matrix remodelling in dental pulp tissue of carious human teeth through the prism of single-cell RNA sequencing
    Item type: Journal Article
    Balic, Anamaria; Perver, Dilara; Pagella, Pierfrancesco; et al. (2023)
    International Journal of Oral Science
    Carious lesions are bacteria-caused destructions of the mineralised dental tissues, marked by the simultaneous activation of immune responses and regenerative events within the soft dental pulp tissue. While major molecular players in tooth decay have been uncovered during the past years, a detailed map of the molecular and cellular landscape of the diseased pulp is still missing. In this study we used single-cell RNA sequencing analysis, supplemented with immunostaining, to generate a comprehensive single-cell atlas of the pulp of carious human teeth. Our data demonstrated modifications in the various cell clusters within the pulp of carious teeth, such as immune cells, mesenchymal stem cells (MSC) and fibroblasts, when compared to the pulp of healthy human teeth. Active immune response in the carious pulp tissue is accompanied by specific changes in the fibroblast and MSC clusters. These changes include the upregulation of genes encoding extracellular matrix (ECM) components, including COL1A1 and Fibronectin (FN1), and the enrichment of the fibroblast cluster with myofibroblasts. The incremental changes in the ECM composition of carious pulp tissues were further confirmed by immunostaining analyses. Assessment of the Fibronectin fibres under mechanical strain conditions showed a significant tension reduction in carious pulp tissues, compared to the healthy ones. The present data demonstrate molecular, cellular and biomechanical alterations in the pulp of human carious teeth, indicative of extensive ECM remodelling, reminiscent of fibrosis observed in other organs. This comprehensive atlas of carious human teeth can facilitate future studies of dental pathologies and enable comparative analyses across diseased organs.
  • Advancing bone biomaterial design: exploring outer microtopography and multi-BSTM membrane production from a single bone source
    Item type: Journal Article
    Özçolak, Birgün; Demir, Öznur; Perver, Dilara; et al. (2025)
    Emergent Materials
    Bone tissue's complex microstructure has inspired the design of biomaterials, yet most approaches focus on replicating its internal structure while neglecting the outer surface topography. This study addresses this gap by developing bone surface topography-mimicked (BSTM) Poly-L-Lactic Acid (PLLA) membranes using by using Polydimethylsiloxane (PDMS) with the soft lithograph technique. Then, bone surface topography mimicked (BSTM) Poly-L-Lactic acid (PLLA) membranes were produced via the solvent casting method, and the procedure was optimized. One hundred BSTM-PLLA membranes were produced from a single bone region using our optimized method. Subsequently, Bone Morphogenic Protein-2 (BMP-2) was loaded as a model growth factor, and diclofenac was loaded as a model drug on the BSTM-PLLA membranes. BSTM-PLLA membranes were modified with collagen type-I (Coll-I) or hydroxyapatite (HAp) to mimic bone`s natural microenvironment more effectively. Surface topography influenced drug release and degradation rates, with BSTM-PLLA membranes exhibiting more controlled release profiles. Characterization studies were followed by cell culture studies using human adipose derived mesenchymal stem cells (ADMSCs). The viability of the ADMSCs on BSTM-PLLA membranes was higher than that of plain PLLA (P-PLLA) membranes. Our findings underscore the potential of BSTM-PLLA membranes for enhanced cell viability, bone regeneration, and drug delivery applications.
  • Single-cell transcriptomics analysis reveals extracellular matrix remodelling in carious human dental pulp
    Item type: Working Paper
    Balic, Anamaria; Perver, Dilara; Pagella, Pierfrancesco; et al. (2023)
    bioRxiv
    The carious lesion is a bacteria caused destruction of tooth mineralized matrices marked by concurrent tissue reparative and immune responses in the dental pulp. While major molecular players in tooth pulp decay have been uncovered, a detailed map of the molecular and cellular landscape of the diseased pulp is still missing. Here we used single-cell RNA sequencing analysis, to generate a comprehensive single-cell atlas of the carious human dental pulp tissue. Our data demonstrated modifications in various cell clusters of the carious pulp, such as immune cells, mesenchymal stem cells (MSC) and fibroblasts, when compared to the healthy dental pulp. These changes include upregulation of genes encoding extracellular matrix (ECM) components and the enrichment of the fibroblast cluster with myofibroblasts. Assessment of the Fibronectin fibres’ mechanical strain showed a significant tension reduction in the carious human pulp, compared to the healthy one. Collectively, the present data demonstrate molecular, cellular and biomechanical alterations in the carious pulp tissue, indicative of extensive ECM remodelling and reminiscent of fibrosis observed in other organs.
Publications 1 - 4 of 4