Journal: Advanced Healthcare Materials

Abbreviation

Adv. Healthcare Mater.

Publisher

Wiley-VCH

Journal Volumes

ISSN

2192-2640
2192-2659

Description

Filters

2013 - 2019242020 - 202537
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Publications 1 - 10 of 61
  • Microfluidics-Driven Manufacturing and Multiscale Analytical Characterization of Nanoparticle-Vesicle Hybrids
    Item type: Journal Article
    Cardellini, Jacopo; Normak, Karl; Gerlt, Michael; et al. (2025)
    Advanced Healthcare Materials
    Coating synthetic nanoparticles (NPs) with lipid membranes is a promising approach to enhance the performance of nanomaterials in various biological applications, including therapeutic delivery to target organs. Current methods for achieving this coating often rely on bulk approaches which can result in low efficiency and poor reproducibility. Continuous processes coupled with quality control represent an attractive strategy to manufacture products with consistent attributes and high yields. Here, this concept is implemented by developing an acoustic microfluidic device together with an analytical platform to prepare nanoparticle-vesicle hybrids and quantitatively characterize the nanoparticle coverage using fluorescence-based techniques at different levels of resolution. With this approach polymethyl methacrylate (PMMA) nanoparticles are successfully coated with liposomes and extracellular vesicles (EVs), achieving a high encapsulation efficiency of 70%. Moreover, the approach enables the identification of design rules to control the efficiency of encapsulation by tuning various operational parameters and material properties, including buffer composition, nanoparticle/vesicle ratio, and vesicle rigidity.
  • Chitosan Electrodeposition for Microrobotic Drug Delivery
    Item type: Journal Article
    Fusco, Stefano; Chatzipirpiridis, George; Sivaraman, Kartik M.; et al. (2013)
    Advanced Healthcare Materials
  • Multifunctional Microflowers for Precise Optoacoustic Localization and Intravascular Magnetic Actuation In Vivo
    Item type: Journal Article
    Nozdriukhin, Daniil; Lyu, Shuxin; Bonvin, Jérôme; et al. (2025)
    Advanced Healthcare Materials
    Efficient drug delivery remains a significant challenge in modern medicine and pharmaceutical research. Micrometer-scale robots have recently emerged as a promising solution to enhance the precision of drug administration through remotely controlled navigation within microvascular networks. Real-time tracking is crucial for accurate guidance and confirmation of target arrival. However, deep-tissue monitoring of microscopic structures in vivo is limited by the sensitivity and spatiotemporal resolution of current bioimaging techniques. In this study, biocompatible microrobots are synthesized by incorporating indocyanine green and iron oxide nanoparticles onto copper phosphate microflowers using a layer-by-layer approach, enhancing optoacoustic contrast and enabling magnetic navigation. Magnetic control of these particles under optoacoustic guidance is demonstrated in vivo. Furthermore, super-resolution optoacoustic imaging, achieved through individual particle tracking, is shown to enable the characterization of microvascular structures and quantification of blood flow. The combination of the microflowers' high carrying capacity, in vivo actuation, and high-resolution tracking capabilities opens new opportunities for precise microvascular targeting and localized administration of theranostic agents via intravascular routes.
  • Perfusable Biohybrid Designs for Bioprinted Skeletal Muscle Tissue
    Item type: Journal Article
    Filippi, Miriam; Yasa, Öncay; Giachino, Jan; et al. (2023)
    Advanced Healthcare Materials
    Engineered, centimeter-scale skeletal muscle tissue (SMT) can mimic muscle pathophysiology to study development, disease, regeneration, drug response, and motion. Macroscale SMT requires perfusable channels to guarantee cell survival, and support elements to enable mechanical cell stimulation and uniaxial myofiber formation. Here, stable biohybrid designs of centimeter-scale SMT are realized via extrusion-based bioprinting of an optimized polymeric blend based on gelatin methacryloyl and sodium alginate, which can be accurately coprinted with other inks. A perfusable microchannel network is designed to functionally integrate with perfusable anchors for insertion into a maturation culture template. The results demonstrate that i) coprinted synthetic structures display highly coherent interfaces with the living tissue, ii) perfusable designs preserve cells from hypoxia all over the scaffold volume, iii) constructs can undergo passive mechanical tension during matrix remodeling, and iv) the constructs can be used to study the distribution of drugs. Extrusion-based multimaterial bioprinting with the inks and design realizes in vitro matured biohybrid SMT for biomedical applications.
  • "Periodontal ligament-on-chip" as a Novel Tool for Studies on the Physiology and Pathology of Periodontal Tissues
    Item type: Journal Article
    Svanberg, Sara; Hirth, Elisabeth; Mitsiadis, Thimios A.; et al. (2024)
    Advanced Healthcare Materials
    Teeth exert fundamental physiological functions, such as mastication and speech, and are a key feature of oral health that affects life quality. Teeth are anchored to the alveolar bone via the periodontal ligament, which provides stability to the teeth and absorbs mechanical stresses during mastication. Periodontal infection leads to periodontitis, a severe inflammation of the supporting soft tissues that ultimately cause tooth loss. Despite the pressing need of periodontal regeneration for improved oral care, efficient in vitro models of the periodontal tissues are still missing, thus hampering the development of novel, faster, and more effective therapy modalities. Herein, a novel "periodontal ligament (PDL)-on-chip" model that integrates patient-derived periodontal ligament cells (PDLCs) and endothelial cells is introduced. This microfluidic platform provides optimal conditions for the formation of extensive and perfusable vascular networks. Furthermore, PDLCs elicit blood vessels' development and maturation while establishing close contacts with the endothelial cells. Potential applications for inflammatory periodontal diseases are also successfully displayed in the "PDL-on-chip" by stimulating inflammation and detecting inflammatory cytokines. This work offers a cornerstone for more complex and specialized microfluidic dental models, which are necessary to unravel complex oral diseases that affect individuals' general health that go beyond the field of dentistry.
  • High-Yield Production of Extracellular Vesicle Subpopulations with Constant Quality Using Batch-Refeed Cultures
    Item type: Journal Article
    Paganini, Carolina; Boyce, Hannah; Libort, Gabriela; et al. (2023)
    Advanced Healthcare Materials
    The conventional manufacturing of extracellular vesicles (EVs) is characterized by low yields and batch-to-batch variability, hampering fundamental research on EVs and their practical applications. Perfusion operations have huge potential to address these limitations and increase the productivity and quality of EVs. In this study, perfusion cultures are simulated with batch-refeed systems and their productivity is compared with that achieved using batch cultures. It is shown that a shift from batch to batch-refeed system can increase the space-time yields of a target EV subpopulation characterized by CD81 and CD63 biomarkers by threefold. Moreover, it is demonstrated that the method facilitates the consistent production of the target EVs from cells maintained under constant conditions for 13 days. These results indicate that the use of perfusion cultures is a promising strategy to increase the manufacturing yield of EVs and control the production of specific EV subpopulations with constant quality attributes, thereby improving reproducibility.
  • A Versatile Approach to Engineering Biomolecule-Presenting Cellular Microenvironments
    Item type: Journal Article
    Lienemann, Philipp S.; Karlsson, Maria; Sala, Ana; et al. (2013)
    Advanced Healthcare Materials
  • Electroforming of Implantable Tubular Magnetic Microrobots for Wireless Ophthalmologic Applications
    Item type: Journal Article
    Chatzipirpiridis, George; Ergeneman, Olgaç; Pokki, Juho; et al. (2015)
    Advanced Healthcare Materials
  • Redox Cycling for Passive Modification of Polypyrrole Surface Properties
    Item type: Journal Article
    Sivaraman, Kartik M.; Özkale, B.; Ergeneman, Olgac; et al. (2013)
    Advanced Healthcare Materials
  • Stretchable Silver Nanowire Microelectrodes for Combined Mechanical and Electrical Stimulation of Cells
    Item type: Journal Article
    Hosseini, Vahid; Gantenbein, Silvan; Avalos Vizcarra, Ima; et al. (2016)
    Advanced Healthcare Materials
Publications 1 - 10 of 61