Yusuke Azuma


Last Name

Azuma

First Name

Yusuke

ORCID

0000-0003-3543-3159

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2018 - 201922020 - 20231
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Publications 1 - 3 of 3
  • Enzyme encapsulation in an engineered lumazine synthase protein cage
    Item type: Book Chapter
    Azuma, Yusuke; Hilvert, Donald (2018)
    Methods in Molecular Biology ~ Protein Scaffolds
  • Complementary charge-driven encapsulation of functional protein by engineered protein cages in cellulo
    Item type: Journal Article
    Zakaszewski, Daniel; Koziej, Lukasz; Pankowski, Jędrzej; et al. (2023)
    Journal of Materials Chemistry B
    Charge-driven inclusion complex formation in live cells was examined using a degradation-prone fluorescent protein and a series of protein cages. The results show that sufficiently strong host-guest ionic interaction and an intact shell-like structure are crucial for the protective guest encapsulation.
  • Cytoplasmic glycoengineering enables biosynthesis of nanoscale glycoprotein assemblies
    Item type: Journal Article
    Tytgat, Hanne L.P.; Lin, Chia-wei; Levasseur, Mikail; et al. (2019)
    Nature Communications
    Glycosylation of proteins profoundly impacts their physical and biological properties. Yet our ability to engineer novel glycoprotein structures remains limited. Established bacterial glycoengineering platforms require secretion of the acceptor protein to the periplasmic space and preassembly of the oligosaccharide substrate as a lipid-linked precursor, limiting access to protein and glycan substrates respectively. Here, we circumvent these bottlenecks by developing a facile glycoengineering platform that operates in the bacterial cytoplasm. The Glycoli platform leverages a recently discovered site-specific polypeptide glycosyltransferase together with variable glycosyltransferase modules to synthesize defined glycans, of bacterial or mammalian origin, directly onto recombinant proteins in the E. coli cytoplasm. We exploit the cytoplasmic localization of this glycoengineering platform to generate a variety of multivalent glycostructures, including self-assembling nanomaterials bearing hundreds of copies of the glycan epitope. This work establishes cytoplasmic glycoengineering as a powerful platform for producing glycoprotein structures with diverse future biomedical applications.
Publications 1 - 3 of 3