Journal: Development

Abbreviation

Development

Publisher

Company of Biologists

Journal Volumes

ISSN

0950-1991
1477-9129

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Publications 1 - 10 of 75
  • DeXtrusion: automatic recognition of epithelial cell extrusion through machine learning in vivo
    Item type: Journal Article
    Villars, Alexis; Letort, Gaelle; Valon, Leo; et al. (2023)
    Development
    Accurately counting and localising cellular events from movies is an important bottleneck of high-content tissue/embryo liv e imaging. Here, we propose a new methodology based on deep learning that allows automatic detection of cellular events and their precise xyt localisation on liv e fluorescent imaging movies without segmentation. We focused on the detection of cell extrusion, the expulsion of dying cells from the epithelial layer, and devised DeXtrusion: a pipeline based on recurrent neural networks for automatic detection of cell extrusion/cell death events in large movies of epithelia mar k e d with cell contour. The pipeline, initially trained on movies of the Drosophila pupal notum marked with fluorescent E-cadherin, is easily trainable, provides fast and accurate extrusion predictions in a large range of imaging conditions, and can also detect other cellular events, such as cell division or cell differentiation. It also performs well on other epithelial tissues with reasonable re-training. Our methodology could easily be applied for other cellular events detected by liv e fluorescent microscopy and could help to democratise the use of deep learning for automatic event detections in developing tissues.
  • Chromatin assembly factor CAF-1 is required for cellular differentiation during plant development
    Item type: Journal Article
    Exner, Vivien; Taranto, Patti; Schönrock, Nicole; et al. (2006)
    Development
  • Polycom group proteins function in the female gametophyte to determine seed development in plants
    Item type: Journal Article
    Leroy, Olivier; Hennig, Lars; Breuninger, Holger; et al. (2007)
    Development
    Polycomb group (PcG) proteins are evolutionary conserved proteins that stably maintain established transcriptional patterns over cell generations. The FERTILIZATION INDEPENDENT SEED (FIS) PcG complex from plants has a similar composition to the Polycomb repressive complex 2 from animals. Mutations in FIS genes cause parent-of-origin-dependent seed abortion. Every seed inheriting a mutant fis allele from the mother is destined to abort,regardless of the presence of a wild-type paternal allele. We tested in Arabidopsis whether the parent-of-origin-dependent seed abortion caused by lack of the FIS subunit MSI1 is caused by parental imprinting of the MSI1 gene. Our data show that MSI1 is not an imprinted gene and that early paternal MSI1 expression is not sufficient to rescue msi1 mutant seeds. By contrast, expression of MSI1 in msi1 female gametophytes is necessary to restore normal seed development, strongly arguing that the female gametophytic effect of fis mutants is caused by a functional requirement for an intact FIS complex in the female gametophyte. Thus, FIS-mediated expression patterns established in the female gametophyte can impact on seed development,establishing fis mutants as true female gametophytic maternal-effect mutants.
  • A quantitative analysis of growth control in the Drosophila eye disc.
    Item type: Journal Article
    Vollmer, Jannik; Fried, Patrick; Sánchez-Aragón, Max; et al. (2016)
    Development
  • Cortical development
    Item type: Journal Article
    Götz, Magdalena; Sommer, Lukas (2005)
    Development
  • URI-1 is required for DNA stability in C. elegans
    Item type: Journal Article
    Parusel, Christine T.; Kritikou, Ekaterini A.; Hengartner, Michael O.; et al. (2006)
    Development
  • SHH propagates distal limb bud development by enhancing CYP26B1-mediated retinoic acid clearance via AER-FGF signalling
    Item type: Journal Article
    Probst, Simone; Kraemer, Conradin; Demougin, Philippe; et al. (2011)
    Development
  • Fibroblast growth factors: key players in regeneration and tissue repair
    Item type: Review Article
    Maddaluno, Luigi; Urwyler, Corinne; Werner, Sabine (2017)
    Development
  • Wollknäuel is required for embryo patterning and encodes the Drosophila ALG5 UDP-glucose
    Item type: Journal Article
    Haecker, Achim; Berman, Mattias; Neupert, Christine; et al. (2008)
    Development
  • Polycomb/Trithorax response elements and epigenetic memory of cell identity.
    Item type: Journal Article
    Ringrose, Leonie; Paro, Renato (2007)
    Development
Publications 1 - 10 of 75