Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals
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Author / Producer
Date
2022
Publication Type
Book Chapter
ETH Bibliography
yes
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Abstract
We describe methods to follow the fate of oligonucleotides after their injection into experimental animals. The quantitation in various tissues, blood or bone marrow cells is possible by chemical ligation PCR. This method works independently of chemical modifications of the oligonucleotide and/or its conjugations to lipid or peptide moieties. Moreover, metabolization intermediates can be detected by mass spectrometry. Together with a readout assay for the biochemical or physiological effects, which will differ, depending on the particular purpose of the oligonucleotide, these methods allow for a comprehensive understanding of oligonucleotide behavior in a living organism.
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Publication status
published
Book title
Alternative Splicing
Journal / series
Volume
2537
Pages / Article No.
335 - 350
Publisher
Humana
Event
Edition / version
Methods
Software
Geographic location
Date collected
Date created
Subject
Oligonucleotide therapeutics; Oligonucleotide conjugates; Oligonucleotide biodistribution; Chemical ligation qPCR; Mass spectometry
Organisational unit
03760 - Hall, Jonathan / Hall, Jonathan