1. Functionalized Polysaccharides Improve Sensitivity of Tyramide/Peroxidase Proximity Labeling Assays through Electrostatic Interactions 

   Heiniger M.; Vanella R.; Walsh-Korb Z.; et al. (2024)

   ACS Biomaterials Science and Engineering

   High-throughput assays that efficiently link genotype and phenotype with high fidelity are key to successful enzyme engineering campaigns. Among these assays, the tyramide/peroxidase proximity labeling method converts the product of an enzymatic reaction of a surface expressed enzyme to a highly reactive fluorescent radical, which labels the cell surface. In this context, maintaining the proximity of the readout reagents to the cell surface ...

   Journal Article

2. Nanopore Deep Sequencing as a Tool to Characterize and Quantify Aberrant Splicing Caused by Variants in Inherited Retinal Dystrophy Genes 

   Maggi J.; Feil S.; Gloggnitzer J.; et al. (2024)

   International Journal of Molecular Sciences

   The contribution of splicing variants to molecular diagnostics of inherited diseases is reported to be less than 10%. This figure is likely an underestimation due to several factors including difficulty in predicting the effect of such variants, the need for functional assays, and the inability to detect them (depending on their locations and the sequencing technology used). The aim of this study was to assess the utility of Nanopore ...

   Journal Article

3. Simulating the Effect of Removing Circulating Tumor Cells (CTCs) from Blood Reveals That Only Implantable Devices Can Significantly Reduce Metastatic Burden of Patients 

   Baumgartner W.; Aceto N.; Lifka S. (2024)

   Cancers

   Circulating tumor cells (CTCs) are cells that have separated from a solid cancerous lesion and entered the bloodstream. They play a crucial role in driving the metastatic spread to distant organs, which is the leading cause of cancer-related deaths. Various concepts for blood purification devices aiming to remove CTCs from the blood and prevent metastases have been developed. Until now, it is not clear if such devices can indeed reduce ...

   Journal Article

4. CXL and the Return of Scale-Up Database Engines 

   Lerner, Alberto; Alonso, Gustavo (2024)

   Proceedings of the VLDB Endowment

   The trend toward specialized processing devices such as TPUs, DPUs, GPUs, and FPGAs has exposed the weaknesses of PCIe in interconnecting these devices and their hosts. Several attempts have been proposed to improve, augment, or downright replace PCIe, and more recently, these efforts have converged into a standard called Compute Express Link (CXL). CXL is already on version 2.0 in terms of commercial availability, but its potential to ...

   Journal Article

5. Enzyme-responsive nanoparticles: enhancing the ability of endolysins to eradicate <i>Staphylococcus aureus</i> biofilm 

   Massani, Mariana Blanco; To, Dennis; Meile, Susanne; et al. (2024)

   Journal of Materials Chemistry B

   Stimuli-responsive nanomaterials show promise in eradicating Staphylococcus aureus biofilm from implants. Peptidoglycan hydrolases (PGHs) are cationic antimicrobials that can be bioengineered to improve the targeting of persisters and drug-resistant bacteria. However, these molecules can be degraded before reaching the target and/or present limited efficacy against biofilm. Therefore, there is an urgent need to improve their potency. ...

   Journal Article

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