Monitoring cell growth of single immobilized S. pombe cells through electrical impedance spectroscopy
Abstract
This work presents a microfluidic system combined with electrical impedance spectroscopy (EIS) for real-time monitoring the growth of fission yeast, S. pombe, at single-cell resolution. Rod-shaped S. pombe cells can be reliably immobilized at orifices in a channel wall by applying mild suction. The system enables subsequent culturing of the immobilized S. pombe cells in an upright position, while longitudinal cell growth can be continuously monitored by means of EIS over several hours and validated through concurrent confocal imaging. Show more
Publication status
publishedBook title
19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)Pages / Article No.
Publisher
Chemical and Biological Microsystems SocietyEvent
Subject
Single-cell analysis; Electrical impedance spectroscopy; Cell trapping; Cell culturingOrganisational unit
03684 - Hierlemann, Andreas / Hierlemann, Andreas
Notes
Extended abstract. Conference lecture on October 26, 2015.More
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ETH Bibliography
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