Monitoring cell growth of single immobilized S. pombe cells through electrical impedance spectroscopy
dc.contributor.author
Zhu, Zhen
dc.contributor.author
Frey, Olivier
dc.contributor.author
Haandbaek, Niels
dc.contributor.author
Franke, Felix
dc.contributor.author
Rudolf, Fabian
dc.contributor.author
Hierlemann, Andreas
dc.date.accessioned
2020-02-21T07:07:12Z
dc.date.available
2017-06-11T23:40:47Z
dc.date.available
2020-02-21T07:07:12Z
dc.date.issued
2015
dc.identifier.isbn
978-0-9798064-8-3
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/111643
dc.description.abstract
This work presents a microfluidic system combined with electrical impedance spectroscopy (EIS) for real-time monitoring the growth of fission yeast, S. pombe, at single-cell resolution. Rod-shaped S. pombe cells can be reliably immobilized at orifices in a channel wall by applying mild suction. The system enables subsequent culturing of the immobilized S. pombe cells in an upright position, while longitudinal cell growth can be continuously monitored by means of EIS over several hours and validated through concurrent confocal imaging.
en_US
dc.language.iso
en
en_US
dc.publisher
Chemical and Biological Microsystems Society
en_US
dc.subject
Single-cell analysis
en_US
dc.subject
Electrical impedance spectroscopy
en_US
dc.subject
Cell trapping
en_US
dc.subject
Cell culturing
en_US
dc.title
Monitoring cell growth of single immobilized S. pombe cells through electrical impedance spectroscopy
en_US
dc.type
Other Conference Item
ethz.book.title
19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2015)
ethz.pages.start
63
en_US
ethz.pages.end
65
en_US
ethz.event
19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (µTAS 2015)
en_US
ethz.event.location
Gyeongju, Korea
en_US
ethz.event.date
October 25-29, 2015
en_US
ethz.notes
Extended abstract. Conference lecture on October 26, 2015.
en_US
ethz.identifier.scopus
ethz.publication.place
Seoul
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03684 - Hierlemann, Andreas / Hierlemann, Andreas
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich::00012 - Lehre und Forschung::00007 - Departemente::02060 - Dep. Biosysteme / Dep. of Biosystems Science and Eng.::03684 - Hierlemann, Andreas / Hierlemann, Andreas
ethz.date.deposited
2017-06-11T23:40:59Z
ethz.source
ECIT
ethz.identifier.importid
imp59365408ef40253047
ethz.ecitpid
pub:173089
ethz.eth
yes
en_US
ethz.availability
Metadata only
en_US
ethz.rosetta.installDate
2017-07-19T08:42:16Z
ethz.rosetta.lastUpdated
2022-03-29T01:02:54Z
ethz.rosetta.versionExported
true
ethz.COinS
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Other Conference Item [18985]