Biotin-independent Strains of Escherichia coli for Enhanced Streptavidin Production
Abstract
Biotin is an archetypal vitamin used as cofactor for carboxylation reactions found in all forms of life. However, biotin biosynthesis is an elaborate multi-enzymatic process and metabolically costly. Moreover, many industrially relevant organisms are incapable of biotin synthesis resulting in the requirement to supplement defined media. Here we describe the creation of biotin-independent strains of Escherichia coli and Corynebacterium glutamicum through installation of an optimized malonyl-CoA bypass, which re-routes natural fatty acid synthesis, rendering the previously essential vitamin completely obsolete. We utilize biotin-independent E. coli for the production of the high-value protein streptavidin which was hitherto restricted because of toxic effects due to biotin depletion. The engineered strain revealed significantly improved streptavidin production resulting in the highest titers and productivities reported for this protein to date. Mehr anzeigen
Persistenter Link
https://doi.org/10.3929/ethz-b-000370994Publikationsstatus
publishedExterne Links
Zeitschrift / Serie
Metabolic EngineeringBand
Seiten / Artikelnummer
Verlag
ElsevierThema
Streptavidin production; Biotin-independent; Bioprocess; Escherichia coliOrganisationseinheit
03602 - Panke, Sven / Panke, Sven
Förderung
289572 - Code-engineered new-to-nature microbial cell factories for novel and safety enhanced bio-production (EC)