Proteomics studies confirm the presence of alternative protein isoforms on a large scale
Open access
Datum
2008Typ
- Journal Article
ETH Bibliographie
yes
Altmetrics
Abstract
Background
Alternative splicing of messenger RNA permits the formation of a wide range of mature RNA transcripts and has the potential to generate a diverse spectrum of functional proteins. Although there is extensive evidence for large scale alternative splicing at the transcript level, there have been no comparable studies demonstrating the existence of alternatively spliced protein isoforms.
Results
Recent advances in proteomics technology have allowed us to carry out a comprehensive identification of protein isoforms in Drosophila. The analysis of this proteomic data confirmed the presence of multiple alternative gene products for over a hundred Drosophila genes.
Conclusions
We demonstrate that proteomics techniques can detect the expression of stable alternative splice isoforms on a genome-wide scale. Many of these alternative isoforms are likely to have regions that are disordered in solution, and specific proteomics methodologies may be required to identify these peptides. Mehr anzeigen
Persistenter Link
https://doi.org/10.3929/ethz-a-005751561Publikationsstatus
publishedExterne Links
Zeitschrift / Serie
Genome BiologyBand
Seiten / Artikelnummer
Verlag
BioMed CentralThema
Alternative splice; Additional data file; Tandem mass spectrum; Alternative splice event; splice isoformsOrganisationseinheit
03663 - Aebersold, Rudolf (emeritus) / Aebersold, Rudolf (emeritus)
02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology
ETH Bibliographie
yes
Altmetrics