Proteomics studies confirm the presence of alternative protein isoforms on a large scale
Open access
Date
2008Type
- Journal Article
ETH Bibliography
yes
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Abstract
Background
Alternative splicing of messenger RNA permits the formation of a wide range of mature RNA transcripts and has the potential to generate a diverse spectrum of functional proteins. Although there is extensive evidence for large scale alternative splicing at the transcript level, there have been no comparable studies demonstrating the existence of alternatively spliced protein isoforms.
Results
Recent advances in proteomics technology have allowed us to carry out a comprehensive identification of protein isoforms in Drosophila. The analysis of this proteomic data confirmed the presence of multiple alternative gene products for over a hundred Drosophila genes.
Conclusions
We demonstrate that proteomics techniques can detect the expression of stable alternative splice isoforms on a genome-wide scale. Many of these alternative isoforms are likely to have regions that are disordered in solution, and specific proteomics methodologies may be required to identify these peptides. Show more
Permanent link
https://doi.org/10.3929/ethz-a-005751561Publication status
publishedExternal links
Journal / series
Genome BiologyVolume
Pages / Article No.
Publisher
BioMed CentralSubject
Alternative splice; Additional data file; Tandem mass spectrum; Alternative splice event; splice isoformsOrganisational unit
03663 - Aebersold, Rudolf (emeritus) / Aebersold, Rudolf (emeritus)
02538 - Institut für Molekulare Systembiologie / Institute for Molecular Systems Biology
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ETH Bibliography
yes
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