Defined conditions for propagation and manipulation of mouse embryonic stem cells
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Datum
2019-03-26Typ
- Journal Article
ETH Bibliographie
no
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Abstract
The power of mouse embryonic stem (ES) cells to colonise the developing embryo has revolutionised mammalian developmental genetics and stem cell research. This power is vulnerable, however, to the cell culture environment, deficiencies in which can lead to cellular heterogeneity, adaptive phenotypes, epigenetic aberrations and genetic abnormalities. Here, we provide detailed methodologies for derivation, propagation, genetic modification and primary differentiation of ES cells in 2i or 2i+LIF media without serum or undefined serum substitutes. Implemented diligently, these procedures minimise variability and deviation, thereby improving the efficiency, reproducibility and biological validity of ES cell experimentation. Mehr anzeigen
Persistenter Link
https://doi.org/10.3929/ethz-b-000346261Publikationsstatus
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Zeitschrift / Serie
DevelopmentBand
Seiten / Artikelnummer
Verlag
Company of BiologistsThema
Differentiation; Embryonic stem cells; Pluripotency; Self-renewalOrganisationseinheit
09658 - von Meyenn, Ferdinand / von Meyenn, Ferdinand
09658 - von Meyenn, Ferdinand / von Meyenn, Ferdinand
ETH Bibliographie
no
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