Open access
Date
2017-10Type
- Review Article
Abstract
Elimination of metabolic flux imbalances in microbial cell factories is an important part in the establishment of viable biotechnological production processes. However, due to the high complexity of cellular metabolism, the limited a priori knowledge about the majority of production pathways and a lack of forward design standards, metabolic engineers strongly rely on empirical screening methodologies to achieve the required improvement of cell behavior. Combinatorial pathway engineering provides an interesting tool to identify global solutions for intricate pathways, but methods for the reduction of combinatorial library size are inevitably required to restrict the experimental effort to an affordable size. Here we review recent advances from this field by scrutinizing commonly applied diversification methods and highlighting crucial strategies for the minimization of experimental effort. Show more
Permanent link
https://doi.org/10.3929/ethz-b-000371013Publication status
publishedExternal links
Journal / series
Current Opinion in BiotechnologyVolume
Pages / Article No.
Publisher
ElsevierOrganisational unit
03602 - Panke, Sven / Panke, Sven
Funding
289326 - Standarization and orthogonalization of the gene expression flow for robust engineering of NTN (new-to-nature) biological properties. (EC)
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