Metadata only
Datum
2020Typ
- Book Chapter
Abstract
In-cell NMR enables structural insights at atomic resolution of proteins in their natural environment. To date, very few methods have been developed to study proteins by in-cell NMR in mammalian systems. Here we describe a detailed protocol to conduct in-cell NMR on the intrinsically disordered protein of alpha-Synuclein (αSyn) in mammalian cells. This chapter includes a simplified expression and purification protocol of recombinant αSyn and its delivery into mammalian cells. The chapter also describes how to assess the cell leakage that might occur to the cells, the setup of the instrument, and how to perform basic analyses with the obtained NMR data. Mehr anzeigen
Publikationsstatus
publishedBuchtitel
Intrinsically Disordered Proteins: Methods and ProtocolsZeitschrift / Serie
Methods in Molecular BiologyBand
Seiten / Artikelnummer
Verlag
Humana PressThema
IDP; Nuclear magnetic resonance; Electroporation; α-synuclein; LeakageOrganisationseinheit
03782 - Riek, Roland / Riek, Roland