Bioprinting of Cartilaginous Auricular Constructs Utilizing an Enzymatically Crosslinkable Bioink
Abstract
Bioprinting of functional tissues could overcome tissue shortages and allow
a more rapid response for treatments. However, despite recent progress in
bioprinting, and its outstanding ability to position cells and biomaterials in a
precise D manner, its success has been limited, due to insucient maturation
of constructs into functional tissue. Here, a novel calcium-triggered enzymatic
crosslinking (CTEC) mechanism for bioinks based on the activation cascade
of Factor XIII is presented and utilized for the biofabrication of cartilaginous
constructs. Hyaluronan transglutaminase (HA-TG), an enzymatically
crosslinkable material, has shown excellent characteristics for chondrogenesis
and builds the basis of the CTEC bioink. The bioink supports tissue maturation
with neocartilage formation and stiening of constructs up to kPa.
Bioprinted constructs remain stable in vivo for weeks and bioprinted
auricular constructs transform into cartilaginous grafts. A major limitation of the
current study is the deposition of collagen I, indicating the maturation toward
fibrocartilage rather than elastic cartilage. Shifting the maturation process
toward elastic cartilage will therefore be essential in order for the developed
bioinks to oer a novel tissue engineered treatment for microtia patients.
CTEC bioprinting furthermore opens up use of enzymatically crosslinkable
biopolymers and their modularity to support a multitude of tissues. Mehr anzeigen
Persistenter Link
https://doi.org/10.3929/ethz-b-000473435Publikationsstatus
publishedExterne Links
Zeitschrift / Serie
Advanced Functional MaterialsBand
Seiten / Artikelnummer
Verlag
Wiley-VCHOrganisationseinheit
03949 - Zenobi-Wong, Marcy / Zenobi-Wong, Marcy
Förderung
173868 - Sinergia Project: A Tissue, Cell and Molecular Approach to Understanding and Treating Microtia (SNF)
Zugehörige Publikationen und Daten
Is supplemented by: https://doi.org/10.3929/ethz-b-000460672