Unraveling virus-induced cellular signaling cascades by label-free quantitative phosphoproteomics
Open access
Datum
2022-03-18Typ
- Journal Article
ETH Bibliographie
yes
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Abstract
Due to the low stoichiometry and highly transient nature of protein phosphorylation it is challenging to capture the dynamics and complexity of phosphorylation events on a systems level. Here, we present an optimized protocol to measure virus-induced phosphorylation events with high sensitivity using label free quantification-based phosphoproteomics. Specifically, we describe filter assisted protein digestion (FASP), enrichment of phosphopeptides, mass spectrometry, and subsequent bioinformatic analysis. For complete details on the use and execution of this protocol, please refer to Hunziker et al. (2022). Mehr anzeigen
Persistenter Link
https://doi.org/10.3929/ethz-b-000527717Publikationsstatus
publishedExterne Links
Zeitschrift / Serie
STAR ProtocolsBand
Seiten / Artikelnummer
Verlag
Cell PressETH Bibliographie
yes
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