Non-FG mediated transport of the large pre-ribosomal subunit through the nuclear pore complex by the mRNA export factor Gle2
dc.contributor.author
Occhipinti, Laura
dc.contributor.author
Chang, Yiming
dc.contributor.author
Altvater, Martin
dc.contributor.author
Menet, Anna M.
dc.contributor.author
Kemmler, Stefan
dc.contributor.author
Panse, Vikram G.
dc.date.accessioned
2020-07-06T13:24:20Z
dc.date.available
2017-06-10T22:29:20Z
dc.date.available
2019-04-02T16:13:00Z
dc.date.available
2020-07-06T13:24:20Z
dc.date.issued
2013-09-01
dc.identifier.issn
1362-4962
dc.identifier.issn
0301-5610
dc.identifier.other
10.1093/nar/gkt675
en_US
dc.identifier.uri
http://hdl.handle.net/20.500.11850/72946
dc.identifier.doi
10.3929/ethz-b-000072946
dc.description.abstract
Multiple export receptors passage bound pre-ribosomes through nuclear pore complexes (NPCs) by transiently interacting with the Phe-Gly (FG) meshwork of their transport channels. Here, we reveal how the non-FG interacting yeast mRNA export factor Gly-Leu-FG lethal 2 (Gle2) functions in the export of the large pre-ribosomal subunit (pre-60S). Structure-guided studies uncovered conserved platforms used by Gle2 to export pre-60S: an uncharacterized basic patch required to bind pre-60S, and a second surface that makes non-FG contacts with the nucleoporin Nup116. A basic patch mutant of Gle2 is able to function in mRNA export, but not pre-60S export. Thus, Gle2 provides a distinct interaction platform to transport pre-60S to the cytoplasm. Notably, Gle2’s interaction platforms become crucial for pre-60S export when FG-interacting receptors are either not recruited to pre-60S or are impaired. We propose that large complex cargos rely on non-FG as well as FG-interactions for their efficient translocation through the nuclear pore complex channel.
en_US
dc.format
application/pdf
en_US
dc.language.iso
en
en_US
dc.publisher
Oxford University Press
en_US
dc.rights.uri
http://creativecommons.org/licenses/by/3.0/
dc.title
Non-FG mediated transport of the large pre-ribosomal subunit through the nuclear pore complex by the mRNA export factor Gle2
en_US
dc.type
Journal Article
dc.rights.license
Creative Commons Attribution 3.0 Unported
dc.date.published
2013-07-31
ethz.journal.title
Nucleic Acids Research
ethz.journal.volume
41
en_US
ethz.journal.issue
17
en_US
ethz.journal.abbreviated
Nucleic Acids Res.
ethz.pages.start
8266
en_US
ethz.pages.end
8279
en_US
ethz.size
14 p.
en_US
ethz.version.deposit
publishedVersion
en_US
ethz.notes
It was possible to publish this article open access thanks to a Swiss National Licence with the publisher
en_US
ethz.identifier.wos
ethz.publication.place
Oxford
en_US
ethz.publication.status
published
en_US
ethz.leitzahl
ETH Zürich::00002 - ETH Zürich, direkt::00012 - Lehre und Forschung, direkt::00007 - Departemente, direkt::02030 - Departement Biologie / Department of Biology::02517 - Institut für Biochemie (IBC) / Institute of Biochemistry (IBC)::03884 - Panse, Vikram G. (SNF-Professur) (ehemalig)
en_US
ethz.leitzahl.certified
ETH Zürich::00002 - ETH Zürich, direkt::00012 - Lehre und Forschung, direkt::00007 - Departemente, direkt::02030 - Departement Biologie / Department of Biology::02517 - Institut für Biochemie (IBC) / Institute of Biochemistry (IBC)::03884 - Panse, Vikram G. (SNF-Professur) (ehemalig)
ethz.date.deposited
2017-06-10T22:31:00Z
ethz.source
ECIT
ethz.identifier.importid
imp593651121f3c217799
ethz.ecitpid
pub:115562
ethz.eth
yes
en_US
ethz.availability
Open access
en_US
ethz.rosetta.installDate
2017-07-14T18:16:29Z
ethz.rosetta.lastUpdated
2024-02-02T11:21:32Z
ethz.rosetta.versionExported
true
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