From single cells to artificial cells: How microfluidics can contribute to a better understanding of cellular processes
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Author
Date
2013Type
- Other Conference Item
ETH Bibliography
yes
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Abstract
Nowadays, microfluidics is an established toolbox for single-cell analysis. The huge potential of microfluidics lies in the advanced ability in handling and positioning of cells, creating chemically-defined liquid environments, and tailoring mechanical or physical conditions. We are developing solutions for the analysis of single cells and single-cell response to external variations (Figure 1). Combinations of microfluidic cell trapping and encapsulation in microchambers accommodating volumes of tens to hundreds of picoliters facilitates analyses of living cells and chemical analysis of cell lysates with unprecedented high sensitivity, which will be demonstrated in several examples.
Living cells are complex systems with numerous processes making it difficult to study a distinct cellular pathway. To overcome these difficulties, we create and employ model membrane systems. These vesicles allow detailed fundamental studies, addressing questions of membrane permeability, membrane fusion and membrane deformation. Show more
Publication status
publishedBook title
17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013)Pages / Article No.
Publisher
Chemical and Biological Microsystems SocietyEvent
Subject
Single-cell analysis; ELISA; Membranes; Vesicles; Artificial cellsOrganisational unit
03807 - Dittrich, Petra / Dittrich, Petra
Notes
Extended abstract. Conference lecture on October 31, 2013.More
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ETH Bibliography
yes
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