Synchrotron based infrared imaging and spectroscopy via focal plane array on live fibroblasts in D2O enriched medium
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Datum
2014-05Typ
- Journal Article
ETH Bibliographie
yes
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Abstract
We successfully tested the viability of using synchrotron-based full-field infrared imaging to study biochemical processes inside living cells. As a model system, we studied fibroblast cells exposed to a medium highly enriched with D2O. We could show that the experimental technique allows us to reproduce at the cellular level measurements that are normally performed on purified biological molecules. We can obtain information about lipid conformation and distribution, kinetics of hydrogen/deuterium exchange, and the formation of concentration gradients of H and O isotopes in water that are associated with cell metabolism. The implementation of the full field technique in a sequential imaging format gives a description of cellular biochemistry and biophysics that contains both spatial and temporal information. Mehr anzeigen
Persistenter Link
https://doi.org/10.3929/ethz-b-000084446Publikationsstatus
publishedExterne Links
Zeitschrift / Serie
Biophysical ChemistryBand
Seiten / Artikelnummer
Verlag
ElsevierThema
Infrared spectromicroscopy; Synchrotron radiation; Single cell; Cell Lipid; Isotope tracer; Full-field IR microscopyETH Bibliographie
yes
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