Microsatellite markers for population studies of the ascomycete Phyllosticta ampelicida, the pathogen causing grape black rot
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Autor(in)
Alle anzeigen
Datum
2014Typ
- Journal Article
ETH Bibliographie
yes
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Abstract
Grape black rot, caused by the homothallic ascomycete Phyllosticta ampelicida, is a disease originating from North America and is widespread in Europe. To investigate population structure and epidemics of this pathogen, we developed 11 microsatellite markers. A multiplex PCR assay was used to amplify genomic DNA from environmental samples including mummified berries and foliar lesions, and from fungal cultures. Environmental samples were collected from five countries (Switzerland, France, Germany, Luxembourg and the USA), and consisted of 64 different genotypes. Five additional genotypes were identified from pure cultures isolated in Switzerland and Germany. The allele rarefaction approach indicated that French vineyards in the region of Bordeaux displayed the greatest mean allelic richness, probably related to the fact that France is the country where the disease was first reported outside North America, in 1885. Our results also suggest the absence of links between the species/cultivar of Vitis hosts and the infecting P. ampelicida genotypes. This is the first report of development of microsatellite markers and their deployment for population studies of P. ampelicida. Mehr anzeigen
Persistenter Link
https://doi.org/10.3929/ethz-b-000098607Publikationsstatus
publishedExterne Links
Zeitschrift / Serie
Phytopathologia mediterraneaBand
Seiten / Artikelnummer
Verlag
Mediterranean Phytopathological UnionThema
Guignardia bidwellii; SSR; population genetics; population structureETH Bibliographie
yes
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